The aim of this work was to determine the importance of the genotype-by-environment interaction in true potato seed breeding. In order to establish the importance of this type of interaction, experiments were sited across three Peruvian locations, San Ramon (warm mid-altitude slopes), Huancayo (cool Andean highlands), and La Molina (coastal desert). The genotype by environment interaction significantly affected tuber yield in all breeding materials (4x-4x from random or selected parents, and 4x-2x crosses). For example, the 10 most outstanding 4x-2x crosses for tuber yield were different when tested in warm and cool locations. Similarly, two clones derived from 4x-4x crosses were among the best 15 hybrids assessed in the two lower altitude locations. These two offspring provide examples of crossover genotype-by-environment interaction for tuber set, non-cross order genotype-by-environment interaction for tuber yield and lack of genotype-by-environment interaction for days to flowering. Because top yielding true potato seed offspring for each location were locally adapted, cultivars or advanced selections from the target area should be the parental sources of true potato seed offspring.