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High-resolution microscopy of cell wall formation in regenerating Mougeotia (Chlorophyceae) protoplasts

Published online by Cambridge University Press:  08 October 2002

LEONIE K. WHIFFEN ,
TERESA P. DIBBAYAWAN  and
ROBYN L. OVERALL
LEONIE K. WHIFFEN
Affiliation:
School of Biological Sciences (A12), The University of Sydney, New South Wales, 2006, Australia
TERESA P. DIBBAYAWAN
Affiliation:
School of Biological Sciences (A12), The University of Sydney, New South Wales, 2006, Australia
ROBYN L. OVERALL
Affiliation:
School of Biological Sciences (A12), The University of Sydney, New South Wales, 2006, Australia
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Abstract

Field emission scanning electron microscopy (FESEM) preparation techniques have been successfully adapted for visualization of the internal and external ultrastructure of Mougeotia filaments and protoplasts. FESEM of the innermost layer of cell wall in Mougeotia filaments revealed that microfibrils are deposited parallel to each other in an interconnected mesh and are oriented perpendicular to the direction of elongation. For the first time, the surface of protoplasts at different stages of regeneration has been observed using FESEM. Nascent microfibril deposition occurs between 1 and 2 h after isolation and arrangement of these microfibrils is random for at least 8 h. Observation of the inner surface of the plasma membrane in burst protoplasts showed that microtubules are not strongly attached for at least 3 h after protoplast isolation.

Keywords

cell wallcellulose microfibrilsfield emission scanning electron microscopyMougeotiamicrotubulesprotoplasts
Type
Research Article
Information
European Journal of Phycology , Volume 37 , Issue 3 , August 2002 , pp. 339 - 347
Copyright
© 2002 British Phycological Society

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