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Alfentanil attenuates phenylephrine-induced contraction in rat aorta

Published online by Cambridge University Press:  01 March 2007

J.-T. Sohn ,
K.-E. Park ,
C. Kim ,
Y.-S. Jeong ,
I.-W. Shin ,
H.-K. Lee  and
Y.-K. Chung
J.-T. Sohn
Affiliation:
Gyeongsang National University College of Medicine, Department of Anesthesia and Pain Medicine, South Korea Institute of Health Sciences, Chilam-dong, Jinju, Gyeongnam, South Korea
K.-E. Park
Affiliation:
Gyeongsang National University College of Medicine, Department of Anesthesia and Pain Medicine, South Korea
C. Kim
Affiliation:
Gyeongsang National University College of Medicine, Department of Anesthesia and Pain Medicine, South Korea
Y.-S. Jeong
Affiliation:
Gyeongsang National University College of Medicine, Department of Anesthesia and Pain Medicine, South Korea
I.-W. Shin
Affiliation:
Gyeongsang National University College of Medicine, Department of Anesthesia and Pain Medicine, South Korea
H.-K. Lee
Affiliation:
Gyeongsang National University College of Medicine, Department of Anesthesia and Pain Medicine, South Korea
Y.-K. Chung
Affiliation:
Gyeongsang National University College of Medicine, Department of Anesthesia and Pain Medicine, South Korea
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Summary

Background and objectives

Alfentanil was reported to relax the rat aorta by direct action on the vascular smooth muscle. The aims of this in vitro study were to examine the effect of alfentanil on phenylephrine-induced contractions in the rat aorta and to determine the cellular mechanism associated with this process.

Methods

Endothelium-denuded aortic rings were suspended in order to record isometric tension. In the rings with or without 10−6 mol naloxone or 10−5 mol verapamil, the concentration–response curves for phenylephrine and potassium chloride were generated in the presence or absence of alfentanil (10−6, 5 × 10−5, 10−4 mol). In the rings exposed to a calcium-free isotonic depolarizing solution, the contractile response induced by the addition of calcium was assessed in the presence or absence of alfentanil (5 × 10−5, 10−4 mol).

Results

Alfentanil (5 × 10−5, 10−4 mol) attenuated (P < 0.05) the phenylephrine-induced contraction in the ring with or without 10−6 mol naloxone but had no effect on the phenylephrine-induced contraction in the rings pretreated with verapamil. Alfentanil (5 × 10−5, 10−4 mol) produced a significant rightward shift (P < 0.01) in the potassium chloride dose–response curve, and attenuated the contractile response (P < 0.001) induced by calcium in the calcium-free isotonic depolarizing solution in a dose-dependent manner.

Conclusions

A supraclinical dose of alfentanil attenuates the phenylephrine-induced contraction via an inhibitory effect on calcium influx by blocking the l-type calcium channels in the rat aortic vascular smooth muscle.

Keywords

ANAESTHETICS INTRAVENOUS, alfentanilAORTA, contraction, smooth muscleADRENERGIC ALPHA-AGONISTS, phenylephrinePOTASSIUM CHLORIDEVERAPAMILRAT
Type
Research Article
Information
European Journal of Anaesthesiology , Volume 24 , Issue 3 , March 2007 , pp. 276 - 282
Copyright
Copyright © European Society of Anaesthesiology 2006

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References

1.Bailey, P, Egan, T. Fentanyl and congeners. In: White, PF, ed. Textbook of Intravenous Anesthesia. Maryland, USA: Williams and Wilkins, 1997: 215–219.Google Scholar
2.Nauta, J, Stanley, TH, de Lange, S, Koopman, D, Spierdijk, J, van Kleef, J. Anaesthetic induction with alfentanil: comparison with thiopental, midazolam, and etomidate. Can J Anaesth 1983; 30: 53–60.CrossRefGoogle ScholarPubMed
3.Kay, B, Cohen, AT. Intravenous anaesthesia for minor surgery. A comparison of etomidate or althesin with fentanyl and alfentanil. Br J Anaesth 1983; 55: 165S–167S.Google ScholarPubMed
4.Bartkowski, RR, McDonnell, TE. Alfentanil as an anesthetic induction agent – a comparison with thiopental–lidocaine. Anesth Analg 1984; 63: 330–334.CrossRefGoogle ScholarPubMed
5.Rucquoi, M, Camu, F. Cardiovascular responses to large doses of alfentanil and fentanyl. Br J Anaesth 1983; 55: 223S–230S.Google ScholarPubMed
6.Vatner, SF. Effects of anesthesia on cardiovascular control mechanisms. Environ Health Perspect 1978; 26: 193–206.Google Scholar
7.Kein, ND, Reitan, JA, White, DA, Wu, CH, JrEisele, JH. Hemodynamic responses to alfentanil in halothane-anesthetized dogs. Anesth Analg 1986; 65: 765–770.Google Scholar
8.White, DA, Reitan, JA, Kein, ND, Thorup, SJ. Decrease in vascular resistance in the isolated canine hindlimb after graded doses of alfentanil, fentanyl, and sufentanil. Anesth Analg 1990; 71: 29–34.CrossRefGoogle ScholarPubMed
9.Karasawa, F, Iwanov, V, Moulds, RFW. Sulfentanil and alfentanil cause vasorelaxation by mechanisms independent of the endothelium. Clin Exp Pharmacol Physiol 1993; 20: 705–711.Google Scholar
10.Godfraind, T, Kaba, A. Blockade or reversal of the contraction induced by calcium and adrenaline in depolarized arterial smooth muscle. Br J Pharmacol 1969; 36: 549–560.CrossRefGoogle ScholarPubMed
11.Karaki, H, Ozaki, H, Hori, M et al. . Calcium movements, distribution, and functions in smooth muscle. Pharmacol Rev 1997; 49: 157–230.Google Scholar
12.Ruegg, UT, Wallnofer, A, Weir, S, Cauvin, C. Receptor-operated calcium-permeable channels in vascular smooth muscle. J Cardiovasc Pharmacol 1989; 14 (Suppl 6): S49–S58.Google Scholar
13.Huang, Y, Ho, IHM. Separate activation of intracellular Ca2+ release, voltage-dependent and receptor-operated Ca2+ channels in the rat aorta. Chin J Physiol 1996; 39: 1–8.Google ScholarPubMed
14.Simpson, AWM, Stampfl, A, Ashley, CC. Evidence for receptor-mediated bivalent-cation entry in A10 vascular smooth-muscle cells. Biochem J 1990; 267: 277–280.Google Scholar
15.Ozdem, SS, Batu, O, Tayfun, F, Yalcin, O, Meiselman, HJ, Baskurt, OK. The effect of morphine in rat small mesenteric arteries. Vascul Pharmacol 2005; 43: 56–61.CrossRefGoogle ScholarPubMed
16.McPherson, RW, Krempasanka, E, Eimerl, D, Traystman, RJ. Effects of alfentanil on cerebral vascular reactivity in dogs. Br J Anaesth 1985; 57: 1232–1238.CrossRefGoogle ScholarPubMed
17.Maitre, PO, Ausems, ME, Vozeh, S, Stanski, DR. Evaluating the accuracy of using population pharmacokinetic data to predict plasma concentrations of alfentanil. Anesthesiology 1988; 68: 59–67.CrossRefGoogle ScholarPubMed
18.Suzer, O, Suzer, A, Aykac, Z, Ozuner, Z. Direct cardiac effects in isolated perfused rat hearts measured at increasing concentrations of morphine, alfentanil, fentanyl, ketamine, etomidate, thiopentone, midazolam and propofol. Eur J Anaesthesiol 1998; 15: 480–485.Google Scholar
19.Flacke, JW, Flacke, WE, Bloor, BC, Olewine, S. Effects of fentanyl, naloxone, and clonidine on hemodynamics and plasma catecholamine levels in dogs. Anesth Analg 1983; 62: 305–313.CrossRefGoogle ScholarPubMed
20.Flacke, JW, Davis, LT, Flacke, WE, Bloor, BC, Van Etten, AP. Effects of fentanyl and diazepam in dogs deprived of autonomic tone. Anesth Analg 1985; 64: 1053–1059.CrossRefGoogle ScholarPubMed
21.Miller, RD, Martineau, RJ, O’Brien, H et al. . Effects of alfentanil on the hemodynamic and catecholamine response to tracheal intubation. Anesth Analg 1993; 76: 1040–1046.Google Scholar
22.Christensen, KL, Mulvany, MJ. Location of resistance arteries. J Vasc Res 2001; 38: 1–12.Google Scholar