Hostname: page-component-76fb5796d-vvkck Total loading time: 0 Render date: 2024-04-26T04:20:48.253Z Has data issue: false hasContentIssue false
  • English
  • Français

Usefulness of ribotyping in a molecular epidemiology study of shigellosis

Published online by Cambridge University Press:  15 May 2009

M. C. Mendoza ,
M. C. Martín  and
M. A. González-Hevia
M. C. Mendoza
Affiliation:
Departamento de Biologia Funcional, Area Microbiología, Universidad de Oviedo; Facultad de Medicina, C/Julián Claveria s/n, 33006-Oviedo, Spain
M. C. Martín
Affiliation:
Departamento de Biologia Funcional, Area Microbiología, Universidad de Oviedo; Facultad de Medicina, C/Julián Claveria s/n, 33006-Oviedo, Spain
M. A. González-Hevia
Affiliation:
Departamento de Biologia Funcional, Area Microbiología, Universidad de Oviedo; Facultad de Medicina, C/Julián Claveria s/n, 33006-Oviedo, Spain Laboratorio de Salud Pública, Principado de Asturias, Oviedo, Spain
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Ribotyping performed with six restriction endonucleases was used to study the molecular epidemiology of shigellosis in Asturias, Spain. The series included Shigella sonnei from 34 sporadic cases, 3 outbreaks and 3 reference strains, and S. flexneri from 13 sporadic cases and 1 reference strain. The S. sonnei strains were grouped into 5 ribotypes with Sal I, 4 with Hind III and Pvu II, 3 with Bgl II and EcoR I and 2 with Hinc II (Discriminatory Index (DI) between 0·54 and 0·14); the S. flexneri into 5 ribotypes with Sal I, Hinc II and Hind III, and 4 with the other enzymes (DI = 0·71–0·63). The combination of results for 2 or more enzymes facilitated an additional discrimination, the highest values in S. sonnei were for the 6 enzymes (16 types, DI = 0·91) and in S. flexneri for some combinations of 3 or more enzymes (7 types, DI = 0·81). Ribotypes with the 6 enzymes defined 16 clonal lines in S. sonnei and 7 in S. flexneri, which showed a different degree of genetic heterogeneity, and all the lines of each species falling into a different cluster. No line appeared as clearly endemic in the bowels of Asturian people.

Type
Research Article
Information
Epidemiology & Infection , Volume 116 , Issue 2 , April 1996 , pp. 127 - 135
Copyright
Copyright © Cambridge University Press 1996

References

1.Stull, TL, LiPuma, JJ, Edlind, TD. A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis 1988; 157: 280–6.CrossRefGoogle ScholarPubMed
2.González-Hevia, MA, Llaneza, J, Mendoza, MC. Usefulness of molecular genetic markers in the typing of Salmonella enterica serovar Enteritidis causing a foodborne outbreak. Internat J Food Microbiol 1994; 22: 97–103.Google Scholar
3.González-Hevia, MA, Mendoza, MC. Differentiation of strains from a food-borne outbreak of Salmonella enterica by phenotypic and genetic typing methods. Eur J Epidemiol 1995; 11: 479–82.CrossRefGoogle ScholarPubMed
4.Hinojosa-Ahumada, M, Swaminathan, B, Hunter, SB, Cameron, DN, Kiehlbauch, IK, Strockbine, NA. Restriction fragment length polymorphisms in rRNA operons for subtyping Shigella sonnei. J Clin Microbiol 1991; 29: 2380–4.CrossRefGoogle ScholarPubMed
5.Faruque, SM, Haider, K, Rahman, MM, Alim, ARM, Ahmad, QS, Albert, MJ, Sack, RB. Differentiation of Shigella flexneri strains by rRNA gene restriction patterns. J Clin Microbiol 1992; 30: 2996–9.CrossRefGoogle ScholarPubMed
6.Nastasi, A, Pignato, S, Mammina, C, Giammanco, G. rRNA gene restriction patterns and biotypes of Shigella sonnei. Epidemiol Infect 1993; 110: 23–30.CrossRefGoogle ScholarPubMed
7.Karaolis, DK, Lan, R, Reeves, P. Sequence variation in Shigella sonnei (Sonnei), a pathogenic clone of Escherichia coli, over four continents and 41 years. J Clin Microbiol 1994; 32: 796–802.CrossRefGoogle ScholarPubMed
8.Mendoza, MC, Mendéz, FJ, Llaneza, J, Gonzalez, AJ, Hardisson, C. Plasmid profile analysis as a tool in characterization of epidemic Shigella sonnei strains. Microbiología SEM 1986; 2: 81–8.Google ScholarPubMed
9.Mendoza, MC, González, AJ, Méndez, FJ, Hardisson, C. Plasmid typing of Shigella sonnei epidemic strains and molecular relationship of their R-plasmids. Eur J Epidemiol 1988; 4: 158–63.CrossRefGoogle ScholarPubMed
10.Deener, HG, Boychuk, I. Species-specific detection of Listeria monocytogenes by DNA amplification. Appl Environ Microbiol 1991, 57: 606–9.CrossRefGoogle Scholar
11.Brosius, J, Ullrich, A, Raker, MA, Gray, A, Dull, TJ, Gutell, RR, Noller, HF. Construction and fine mapping of recombinant plasmid containing the rrnB ribosomal RNA operon of E. coli. Plasmid 1981; 6: 112–8.CrossRefGoogle ScholarPubMed
12.Sambrook, J, Fritch, EF, Maniatis, T. Molecular cloning: A laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1989.Google Scholar
13.Hunter, PR, Gaston, MA: Numerical index of the discriminatory ability of typing systems: an application of Simpson' index of diversity. J Clin Microbiol 1988; 26: 2465–6.CrossRefGoogle ScholarPubMed
14.Millemann, Y, Lesage, MC, Chaslus-Dancla, E, Lafont, JP. Values of plasmid profiling, ribotyping and detection of IS200 for tracing avian isolates of Salmonella typhimurium and S. enteritidis. J Clin Microbiol 1995; 33: 173–9.CrossRefGoogle ScholarPubMed
15.Priest, F, Austin, B. Numerical taxonomy. In Priest, F, Austin, B, Modern bacterial taxonomy, 2nd ed. London: Chapman & Hall, 1993: 14–49.Google Scholar
16.Lee, LA, Shapiro, CN, Hargrett-Bean, N, Tauxe, RV. Hyperendemic shigellosis in the United States: a review of surveillance data for 1967–1988. J Infect Dis 1991; 164: 894–900.CrossRefGoogle ScholarPubMed
17.Public Health Laboratory Service. A foodborne outbreak of Shigella sonnei infection in Europe. Communicable Dis Rep 4: 25.Google Scholar
18.Kapperud, G, Rorvik, LM, Hasseltvedt, V et al. Outbreak of Shigella sonnei infection traced to imported iceberg lettuce. J Clin Microbiol 1995; 66: 609–14.CrossRefGoogle Scholar
19.Morera, MA, Espejo, E, Coll, P et al. Brote epidemico de shigelosis por ingesta de agua. Enfem Infec Microbiol Clin 1995; 13: 160–5.Google Scholar
20.Reeve, G, Martin, DL, Pappas, J, Thompson, RE, Greene, KD. An outbreak of shigellosis associated with the consumption of raw oysters. N Eng J Med 1989; 321: 224–7.CrossRefGoogle ScholarPubMed
21.Lermam, Y, Yavzori, M, Ambar, R, Sechter, I, Wiener, M, Cohen, D. Epidemic spread of Shigella sonnei shigellosis and evidence for development of immunity among children attending day-care centers in a communal settlement (kibbutz). J Clin Microbiol 1994; 32: 1092–4.CrossRefGoogle Scholar
22. Boletín Epidemiológico y Microbiológico, Spain, 1993, vol. I 2: 21–40.Google Scholar