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The use of a radial haemolysis test for neuraminidase antibodies in the diagnosis of influenza A infection

Published online by Cambridge University Press:  19 October 2009

E. A. Grilli  and
A. J. Smith
E. A. Grilli
Affiliation:
Influenza Research Unit, Public Health Laboratory, St Luke's Hospital, Guildford, Surrey GU1 3NT
A. J. Smith
Affiliation:
Influenza Research Unit, Public Health Laboratory, St Luke's Hospital, Guildford, Surrey GU1 3NT
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Summary

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A radial haemolysis test designed to detect antibody specifically directed against the neuraminidase is described and compared with currently available techniques for the routine diagnosis of infection with influenza viruses.

Paired sera were available from two outbreaks of influenza A, one with the N1N1 subtype and the other with the H3N2 subtype. This technique confirmed diagnosis in 92% of cases diagnosed by other laboratory methods.

Type
Research Article
Information
Journal of Hygiene , Volume 91 , Issue 1 , August 1983 , pp. 147 - 156
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Aymard-Henry, M., Coleman, M. T., Dowdle, W. R., Laver, W. G., Schild, G. C. & Webster, R. G. (1973). Influenza neuraminidase and neuraminidase inhibition test procedures. Bulletin of the World Health Organisation 48, 199–202.Google ScholarPubMed
Bradstreet, C. M. P. & Taylor, C. E. D. (1962). Technique of complement fixation test applicable to the diagnosis of virus diseases. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 21, 96–104.Google Scholar
Callow, K. A. & Beare, A. S. (1976). Measurement of antibody to influenza virus neuraminidase by single radial haemolysis in agarose gels. Infection and Immunity 13, 1–8.CrossRefGoogle ScholarPubMed
Chakraverty, P. (1980). Comparison of haemagglutination inhibition and single radial haemolysis techniques for the detection of antibodies to influenza B virus. Archives of Virology 63, 285–289.CrossRefGoogle ScholarPubMed
Grilli, E. A. & Davies, J. R. (1981). Influenza B at Christ's Hospital: natural antibody to influenza B estimated by radial haemolysis. Journal of Hygiene 87, 211–218.CrossRefGoogle ScholarPubMed
Kendal, A. P., Minuse, E. & Davenport, F. M. (1972). An improved procedure for measuring neuraminidase antibodies by haemagglutination-inhibition. Zeitschrift für Naturforschung 27b, 241–245.CrossRefGoogle Scholar
Oxford, J. S. (1982). The use of monoclonal antibodies in virology. Journal of Hygiene 88, 301–308.CrossRefGoogle ScholarPubMed
Oxford, J. S., Schild, G. C., Potter, C. W. & Jennings, R. (1979). The specificity of the anti-haemagglutinin antibody response induced in man by inactivated influenza vaccines and by natural infection. Journal of Hygiene 82, 51–61.CrossRefGoogle Scholar
Palmer, D. F., Coleman, M. T., Dowdle, W. R. & Schild, G. C. (1975). Haemagglutination inhibition test. In Advanced Laboratory Techniques for Influenza Diagnosis, pp. 20–45. Atlanta: U.S. Department of Health, Education and Welfare.Google Scholar
Schild, G. C., Pereira, M. S. & Chakraverty, P. (1975). Single radial haemolysis: a new method for the assay of antibody to influenza haemagglutinin. Bulletin of the World Health Organisation 52, 43–50.Google ScholarPubMed
Smith, A. J. (1977). Natural Infection and Vaccination with Influenza A (H3N2). Ph.D. thesis: University of Surrey.Google Scholar
Smith, A. J. & Davies, J. R. (1976). Natural infection with influenza A (H3N2). The development, persistence and effect of antibodies to the surface antigens. Journal of Hygiene 77, 271–282.CrossRefGoogle Scholar