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The transmission dynamics of Campylobacter jejuni among broilers in semi-commercial farms in Jordan

  • M. I. Neves (a1), I. Malkawi (a2), M. Walker (a1), A. Alaboudi (a2), E. Abu-Basha (a2), D. P. Blake (a1), J. Guitian (a1) and M. Crotta (a1)...

Abstract

Campylobacter is the leading cause of foodborne bacterial gastroenteritis in humans worldwide, often associated with the consumption of undercooked poultry. In Jordan, the majority of broiler chicken production occurs in semi-commercial farms, where poor housing conditions and low bio-security are likely to promote campylobacter colonisation. While several studies provided estimates of the key parameters describing the within-flock transmission dynamics of campylobacter in typical high-income countries settings, these data are not available for Jordan and Middle-East in general. A Bayesian model framework was applied to a longitudinal dataset on Campylobacter jejuni infection in a Jordan flock to quantify the transmission rate of C. jejuni in broilers within the farm, the day when the flock first became infected, and the within-flock prevalence (WFP) at clearance. Infection with C. jejuni is most likely to have occurred during the first 8 days of the production cycle, followed by a transmission rate value of 0.13 new infections caused by one infected bird/day (95% CI 0.11–0.17), and a WFP at clearance of 34% (95% CI 0.24–0.47). Our results differ from published studies conducted in intensive poultry production systems in high-income countries but are well aligned with the expectations obtained by means of structured questionnaires submitted to academics with expertise on campylobacter in Jordan. This study provides for the first time the most likely estimates and credible intervals of key epidemiological parameters driving the dynamics of C. jejuni infection in broiler production systems commonly found in Jordan and the Middle-East and could be used to inform Quantitative Microbial Risk Assessment models aimed to assess the risk of human exposure/infection to campylobacter through consumption of poultry meat.

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Copyright

This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

Corresponding author

Author for correspondence: M. I. Neves, E-mail: mneves@rvc.ac.uk

References

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1.EFSA (2010) Scientific opinion on quantification of the risk posed by broiler meat to human campylobacteriosis in the EU. EFSA Journal 8, 1437.
2.EFSA (2011) Scientific opinion on campylobacter in broiler meat production: control options and performance objectives and/or targets at different stages of the food chain. EFSA Journal 9, 2015.
3.Seriwatana, J et al. (1988) Foods as a source of enteropathogens causing childhood diarrhea in Thailand. The American Journal of Tropical Medicine and Hygiene 39, 97102.
4.Osano, O and Arimi, SM (1999) Retail poultry and beef as sources of Campylobacter jejuni. East African Medical Journal 76, 141143.
5.Hafez, AA et al. (2018) Prevalence, cytotoxicity and antibiotic susceptibility of Campylobacter species recovered from retail chicken meat in Mansoura, Egypt. African Journal of Microbiology Research 12, 501507.
6.Department of Statistics. Data by sector. Available at http://web.dos.gov.jo/ (Accessed April 2017).
7.FAO. Poultry sector country review: Jordan 2007. Available at http://www.fao.org/3/a-ai322e.pdf (Accessed April 2017).
8.Osaili, TM, Alaboudi, AR and Al-Akhras, RR (2012) Prevalence and antimicrobial susceptibility of Campylobacter spp. in live and dressed chicken in Jordan. Foodborne Pathogens and Disease 9, 5458.
9.Cawthraw, SA et al. (1996) Increased colonization potential of Campylobacter jejuni strain 81116 after passage through chickens and its implication on the rate of transmission within flocks. Epidemiology and Infection 117, 213215.
10.Newell, DG and Fearnley, C (2003) Sources of Campylobacter colonization in broiler chickens. Applied Environmental Microbiology 69, 43434351.
11.Ringoir, D (2003) Colonisation phenotype and colonisation potential differences in Campylobacter jejuni strains in chickens before and after passage in vivo. Veterinary Microbiology 92, 225235.
12.Wassenaar, TM et al. (1993) Colonization of chicks by motility mutants of Campylobacter jejuni demonstrates the importance of flagellin A expression. Journal of General Microbiology 139 Pt 6, 11711175.
13.Newell, DG (2002) The ecology of Campylobacter jejuni in avian and human hosts and in the environment. International Journal of Infectious Diseases 6(suppl. 3), 3S163S20; discussion 3S20-1, 3S53-8.
14.Evans, S and Sayers, A (2000) A longitudinal study of campylobacter infection of broiler flocks in Great Britain. Preventive Veterinary Medicine 46, 209223.
15.Lindblom, GB, Sjörgren, E and Kaijser, B (1986) Natural campylobacter colonisation in chickens raised under different environmental conditions. The Journal of Hygiene 96, 385391.
16.Jacobs-Reitsma, WF et al. (1995) Epidemiology of Campylobacter spp. at two Dutch broiler farms. Epidemiology and Infection 114, 413.
17.Gregory, E et al. (1997) Epidemiological study of Campylobacter spp. in broilers: source, time of colonisation, and prevalence. Avian Diseases 41, 890898.
18.Van Gerwe, T et al. (2005) Quantifying transmission of Campylobacter spp. among broilers. Applied and Environmental Microbiology 71, 57655770.
19.Crotta, M, Georgiev, M and Guitian, J (2017) Quantitative risk assessment of Campylobacter in broiler chickens – assessing interventions to reduce the level of contamination at the end of the rearing period. Food Control 75, 2939.
20.Katsma, WEA et al. (2007) Assessing interventions to reduce the risk of Campylobacter prevalence in broilers. Risk Analysis 27, 863876.
21.Goddard, AD et al. (2014) Estimating the time at which commercial broiler flocks in Great Britain become infected with Campylobacter: a Bayesian approach. Epidemiology and Infection 142, 18841892.
22.Van Gerwe, T et al. (2009) Quantifying transmission of Campylobacter jejuni in commercial broiler flocks. Applied and Environmental Microbiology 75, 625628.
23.Matt, M et al. (2016) Estimating sensitivity and specificity of a PCR for boot socks to detect Campylobacter in broiler primary production using Bayesian latent class analysis. Preventive Veterinary Medicine 128, 5157.
24.Ringoir, DD, Szylo, D and Korolik, V (2007) Comparison of 2-day-old and 14-day-old chicken colonization models for Campylobacter jejuni. FEMS Immunology and Medical Microbiology 49, 155158.
25.Cawthraw, SA and Newell, DG (2010) Investigation of the presence and protective effects of maternal antibodies against Campylobacter jejuni in chickens. Avian Diseases 54, 8693.
26.Sahin, O et al. (2003) Effect of Campylobacter-specific maternal antibodies on Campylobacter jejuni colonization in young chickens. Applied and Environmental Microbiology 69, 53725379.
27.ISO 10272-1:2006. Microbiology of food and animal feeding stuffs: horizontal method for detection and enumeration of Campylobacter spp. Part 1: Detection method. Available at https://www.iso.org/standard/37091.html.
28.Nayak, R, Stewart, TM and Nawaz, MS (2005) PCR identification of Campylobacter coli and Campylobacter jejuni by partial sequencing of virulence genes. Molecular and Cellular Probes 19, 187193.
29.Hartnett, E et al. (2001) A quantitative risk assessment for the occurrence of campylobacter in chickens at the point of slaughter. Epidemiology and Infection 127, 195206.
30.Achen, M, Morishita, TY and Ley, EC (1998) Shedding and colonisation of Campylobacter jejuni in broilers from day-of-hatch to slaughter age. Avian Diseases 42, 732737.
31.Plummer, M. Working Papers JAGS: a program for analysis of Bayesian graphical models using Gibbs sampling. Available at https://www.rproject.org/conferences/DSC2003/Proceedings/Plummer.pdf (Accessed August 2017).
32.R Development Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria, 2008. ISBN 3-900051-07-0 U. Available at https://www.r-project.org (Accessed August 2017).
33.Hamra, G, MacLehose, R and Richardson, D (2013) Markov Chain Monte Carlo: an introduction for epidemiologists. International Journal of Epidemiology 42, 627634.
34.Ishihara, K et al. (2006) The dynamics of antimicrobial-resistant Campylobacter jejuni on Japanese broiler farms. The Journal of Veterinary Medical Science 68, 515518.
35.Line, JE (2006) Influence of relative humidity on transmission of Campylobacter jejuni in broiler chickens. Poultry Science 85, 11451150.
36.Korolik, V et al. (1998) Isolation and molecular analysis of colonising and non-colonising strains of Campylobacter jejuni and Campylobacter coli following experimental infection of young chickens. Veterinary Microbiology 60, 239249.
37.Chaloner, G et al. (2014) Dynamics of dual infection with Campylobacter jejuni strains in chickens reveals distinct strain-to-strain variation in infection ecology. Applied and Environmental Microbiology 80, 63666372.
38.Konkel, ME et al. (2007) Campylobacter jejuni strains compete for colonisation in broiler chicks. Applied and Environmental Microbiology 73, 22972305.
39.Haag, LM et al. (2012) Intestinal microbiota shifts towards elevated commensal Escherichia coli loads abrogate colonization resistance against Campylobacter jejuni in mice. PLoS ONE 7, e35988.
40.Stanley, D et al. (2013) Highly variable microbiota development in the chicken gastrointestinal tract. PLoS ONE 8, e84290.
41.Pandit, RJ et al. (2018) Microbial diversity and community composition of caecal microbiota in commercial and indigenous Indian chickens determined using 16s rDNA amplicon sequencing. Microbiome 6, 115.
42.Bull, SA et al. (2006) Sources of campylobacter spp. Colonizing housed broiler flocks during rearing. Applied and Environmental Microbiology 72, 645652.
43.Georgiev, M, Beauvais, W and Guitian, J (2017) Effect of enhanced biosecurity and selected on-farm factors on campylobacter colonization of chicken broilers. Epidemiology and Infection 145, 553567.
44.Stern, NJ and Meinersmann, RJ (1989) Potentials for colonization control of Campylobacter-jejuni in the chicken. Journal of Food Protection 52, 427.

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