Hostname: page-component-848d4c4894-pjpqr Total loading time: 0 Render date: 2024-07-08T05:09:59.091Z Has data issue: false hasContentIssue false
  • English
  • Français

Susceptibility of the LLC-MK2 line of monkey kidney cells to human enteroviruses

Published online by Cambridge University Press:  15 May 2009

M. H. Hambling  and
Patricia M. Davis
M. H. Hambling
Affiliation:
Virus Reference Laboratory, Central Public Health Laboratory Colindale, London, N.W. 9
Patricia M. Davis
Affiliation:
Virus Reference Laboratory, Central Public Health Laboratory Colindale, London, N.W. 9
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The relative susceptibility of the LLC-MK2 cell line and primary rhesus monkey kidney cultures to thirty-eight prototype strains of human enteroviruses is described; of these strains only ECHO types 16, 18, 21 and 23 failed to cause CPE in the continuous cell line. The efficiency of the two tissues for the isolation of enteroviruses from faecal extracts is compared. The results show that the LLC-MK2 cell line is very satisfactory for the isolation of Coxsackie B and polioviruses, but not so useful for the isolation of ECHO viruses. The identification of enteroviruses by neutralization tests in LLC-MK2 cells is successful.

Type
Research Article
Information
Journal of Hygiene , Volume 63 , Issue 2 , June 1965 , pp. 169 - 174
Copyright
Copyright © Cambridge University Press 1965

References

Hambling, M. H., Davis, P. M. & Macrae, A. D. (1963). The typing of enteroviruses in tissue culture by neutralization with composite antiserum pools. J. Hyg., Camb., 61, 479.Google ScholarPubMed
Hull, R. N., Cherry, W. R. & Johnson, I. S. (1956). The adaption and maintenance of mammalian cells to continuous growth in tissue culture. Anat. Rec. 124, 490.Google Scholar
Hull, R. N., Cherry, W. R. & Tritch, O. J. (1962). Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2 (NCTC-3196) and their utility in virus research. J. exp. Med. 115, 903.CrossRefGoogle ScholarPubMed
HsiungG.,-D. G.,-D. (1962). Further studies on characterization and grouping of ECHO viruses. Ann. N.Y. Acad. Sci. 101, 413.CrossRefGoogle ScholarPubMed
Melnick, J. L. (1957). Role of tissue cultures in the isolation and study of viruses: ECHO viruses. Spec. Publ. N. Y. Acad. Sci. 5, 365.Google Scholar
Pal, S. R., McQuillin, J. & Gardner, P. S. (1963). A comparative study of susceptibility of primary monkey kidney cells, Hep 2 cells and HeLa cells to a variety of faecal viruses. J. Hyg., Camb., 61, 493.Google ScholarPubMed
Rappaport, C. (1956). Trypsinization of monkey kidney tissue: an automatic method for the preparation of cell suspensions. Bull. World Hlth Org. 14, 147.Google ScholarPubMed
Reed, L. J. & Muench, H. (1938). A simple method of estimating fifty per cent endpoints. Amer. J. Hyg. 27, 493.Google Scholar
Tobin, J. O'H. (1960). Notes on viruses likely to be encountered in vaccine production using monkey kidney material. Bull. World Hlth Org. 22, 431.Google ScholarPubMed