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A study of enterotoxigenic Escherichia coli, serogroup 0126, by bacterial restriction endonuclease DNA analysis (BRENDA)

Published online by Cambridge University Press:  19 October 2009

R. B. Marshall ,
P. J. Winter ,
A. J. Robinson  and
K. A. Bettelheim
R. B. Marshall
Affiliation:
Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand
P. J. Winter
Affiliation:
Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand
A. J. Robinson
Affiliation:
M.R.C. Virus Research Unit, University of Otago, P.O. Box 56, Dunedin, New Zealand
K. A. Bettelheim
Affiliation:
National Health Institute, Porirua, New Zealand
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Summary

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Sixteen isolates of Escherichia coli were subjected to bacterial restriction endonuclease DNA analysis (BRENDA). Nine of these isolates were from an outbreak of human diarrhoea and produced stable toxin, the remaining seven were non-toxigenic strains from animal and human sources. The isolates from the outbreak produced indistinguishable DNA electrophoretic patterns in spite of their assignment to seven different H serotypes. Their BRENDA patterns were markedly different from the other isolates examined. These results support the epidemiological evidence that a single-strain outbreak had occurred, and they cast doubt on the value of H typing for this particular investigation.

Type
Research Article
Information
Journal of Hygiene , Volume 94 , Issue 3 , June 1985 , pp. 263 - 268
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Berry, R. J., Bettelheim, K. A. & Gracey, M. (1983). Studies on enterotoxigenic Escherichia coli isolated from persons without diarrhoea in Western Australia.' Journal of Hygiene 90 99–106.CrossRefGoogle ScholarPubMed
Bettelheim, K. A. & Reeve, K. G. (1982). An outbreak of gastroenteritis due to enteropathogenic Escherichia coli, which are also enterotoxigenic. New Zealand Medical Journal 95 215–216.Google ScholarPubMed
Bettelheim, K. A., Wilson, M. W., Shooter, R. A. & O'Farrell, S. M. (1980). Studies on the enterotoxigenicity of environmental Escherichia coli belonging to serotypes normally considered enterotoxigenic. Journal of Hygiene 84, 411–414.CrossRefGoogle ScholarPubMed
Bradbury, W. C, Pearson, A. D., Marko, M. A., Congi, Rosa V. & Penner, J. L. (1984). Investigation of Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis. Journal of Clinical Microbiology 19, 342–346.CrossRefGoogle ScholarPubMed
Eckhardt, T. (1978). A rapid method for identification of plasmid desoxyribonueleic acid in bacteria. Plasmid 1, 584–588.CrossRefGoogle ScholarPubMed
Fredericq, P., Betz-Bareau, M. & Nocolle, P. (1956). Typage de souches d'Ecoli de gastro-entérite infantile par recherche de leurs propriétés colicinogènes. Comptes Rendus des Séances de la Société de Biologie et de ses filiales(Paris) 150, 2039–2042.Google Scholar
Giannella, R. A. (1976). Suckling mouse model for detection of heat stable Escherichia coli enterotoxin: characteristics of the model. Infection and Immunity 14, 95–99.CrossRefGoogle ScholarPubMed
Goldschmid, M. C. & Dupont, H. L. (1976). Enteropathogenic Escherichia coli: lack of correlation of serotype with pathogenicity. Journal of Infectious Diseases 133, 153–156.CrossRefGoogle Scholar
Kakoyiannis, C. K., Winter, P. J. & Marshall, R. B. (1984). Identification of isolates of Campylobacter coli from animals and humans by bacterial restriction endonuclease DNA analysis (BRENDA). Applied and Environmental Microbiology 48, 545–549.CrossRefGoogle Scholar
Kaper, J. B., Bradford, H. B., Roberts, H. C. & Falkow, S. (1982). Molecular epidemiology of vibrio cholera in the U.S. Gulf Coast. Journal of Clinical Microbiology 16, 129–134.CrossRefGoogle ScholarPubMed
Kelly, T. J. & Smith, H. O. (1970). A restriction enzyme from Haemophilus influenzae. II. Base sequence of the recognition site. Journal of Molecular Biology 51, 393–409.CrossRefGoogle Scholar
Le Pecq, J. B. & Paoletti, C. (1966). A new fluorometric method for RNA and DNA determination. Analytical Biochemistry 17, 100.CrossRefGoogle ScholarPubMed
Marshall, R. B., Wilton, B. E. & Robinson, A. J. (1981). Identification of Leptospira serovars by restriction-endonuclease analysis. Journal of Medical Microbiology 14, 163–166.CrossRefGoogle ScholarPubMed
Merson, M. H., Rowe, B., Black, R. E., Huq, I., Glass, R. I. & Eusof, A. (1980). Use of antisera for identification of enterotoxigenic Escherichia coli. Lancet ii, 222–224.CrossRefGoogle Scholar
Nathans, D. & Smith, H. O. (1975). Restriction endonucleases in the analysis and restructuring of DNA molecules. Annual Review of Biochemistry 44, 273–293.CrossRefGoogle ScholarPubMed
Robinson, A. J., Ramadass, P., Lee, A. & Marshall, R. B. (1982). Differentiation of subtypes within Leptospira interrogans serovars hardjo, balcanica and tarassovi by bacterial restriction endonuclease DNA analysis (BRENDA). Journal of Medical Microbiology 15, 331–338.CrossRefGoogle ScholarPubMed
Sack, D. & Sack, R. B. (1975). Test for enterotoxigenic Escherichia coli using Y1 adrenal cells in miniculture, Infection and Immunity 11, 334–336.CrossRefGoogle ScholarPubMed
Sadowski, P. L., Peterson, B. C., Gerding, D. N. & Cleary, P. P. (1979). Physical characterisation of ten R plasmids obtained from an outbreak of nosocomial Klebsiella pneumoniae infections. Antimicrobial Agents and Chemotherapy 15, 616–624.CrossRefGoogle Scholar
Scotland, S. M., Day, H. P., Cravioto, A., Thomas, L. V. & Rowe, B. (1981). Production of heat-labile or heat-stable enterotoxins by strains of Escherichia coli belonging to serogroups of 044, 0 114 and 0 128. Infection and Immunity 31, 500–503.CrossRefGoogle ScholarPubMed