Hostname: page-component-848d4c4894-8kt4b Total loading time: 0 Render date: 2024-06-24T16:49:41.901Z Has data issue: false hasContentIssue false
  • English
  • Français

Studies on vaccinia haemagglutinin: II. Some immunological properties

Published online by Cambridge University Press:  15 May 2009

C. M. Chu
C. M. Chu
Affiliation:
Department of Pathology, University of Cambridge
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Vaccinated rabbits develop serum anti-haemagglutinin after infection. The anti-haemagglutinin is not found to any appreciable titre in normal human and animal sera tested. It is present in the globulin fraction of rabbit serum and is stable at 70° C. for 30 min. but destroyed at 80° C. for 30 min.

2. Observations over a period of 6 months of variations in serum antibodies in vaccinated rabbits demonstrate that whereas the agglutinin, precipitin and complement-fixing antibody varied in parallel with each other, the anti-haemagglutinin varied independently and in general remained more or less steady throughout the period.

3. Some experimental observations of the haemagglutinin anti-haemagglutinin reaction are reported. The haemagglutinin inactivated by immune serum can be reactivated by boiling. Haemagglutinin adsorbed on to red cells can be eluted by subsequent addition of immune serum.

4. Failure to obtain complement fixation with the haemagglutinin anti-haemagglutinin system is recorded.

5. Vaccinia haemagglutinin is distinct from the L-S antigen.

6. By absorption test, it has been shown that the anti-haemagglutinin is a distinct antibody related neither to L or S antibodies nor to neutralizing antibody.

7. It is concluded that vaccinia haemagglutinin represents a new and distinct antigen occurring in a relatively large particulate state in vaccinia infected tissues. The possible theoretical implications of such findings are discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 46 , Issue 1 , March 1948 , pp. 49 - 59
Copyright
Copyright © Cambridge University Press 1948

References

REFERENCES

Andrewes, C. H. & Elford, W. J. (1933). Brit. J. Exp. Path. 14, 367.Google Scholar
Burnet, F. M. & Boake, W. C. (1946). J. Immunol. 53, 1.CrossRefGoogle Scholar
Burnet, F. M. & Stone, J. D. (1946). Aust. J. Exp. Biol. Med. Sci. 24, 1.CrossRefGoogle Scholar
Claude, A. (1941). Cold Spr. Harb. Symp. Quant. Biol. 9, 263.CrossRefGoogle Scholar
Craigie, J. (1932). Brit. J. Exp. Path. 13, 259.Google Scholar
Craigie, J. & Wishart, F. O. (1934). Brit. J. Exp. Path. 15, 390.Google Scholar
Craigie, J. & Wishart, F. O. (1936 a). J. Exp. Med. 64, 803.CrossRefGoogle Scholar
Craigie, J. & Wishart, F. O. (1936 b). J. Exp. Med. 64, 819.CrossRefGoogle Scholar
Cromartie, W. J., Watson, D. W., Bloom, W. L. & Heckley, R. J. (1946). J. Infect. Dis. 80, 14.CrossRefGoogle Scholar
Curnen, E. C. & Horsfall, F. L. (1946). J. Exp. Med. 83, 105.CrossRefGoogle Scholar
Davis, G. E. (1931). Amer. J. Hyg. 13, 79.Google Scholar
Gladstone, G. P. (1946). Brit. J. Exp. Path. 27, 394.Google Scholar
Hughes, T. P. (1933). J. Immunol. 25, 275.CrossRefGoogle Scholar
Keogh, E. V. (1933). J. Path. Bact. 43, 441.CrossRefGoogle Scholar
Kidd, J. G. (1946). Cold Spr. Harb. Symp. Quant. Biol. 11, 93.Google Scholar
Ledingham, J. C. G., Morgan, W. T. J. & Petrie, G. F. (1931). Brit. J. Exp. Path. 12, 357.Google Scholar
Nagler, F. P. O. (1942). Med. J. Aust. 1, 281.CrossRefGoogle Scholar
Nagler, F. P. O. (1944). Aust. J. Exp. Biol. Med. Sci. 22, 29.CrossRefGoogle Scholar
Salaman, M. H. (1934). Brit. J. Exp. Path. 15, 381.Google Scholar
Salaman, M. H. (1937). Brit. J. Exp. Path. 18, 245.Google Scholar
Salaman, M. H. (1938). Brit. J. Exp. Path. 19, 192.Google Scholar
Smadel, J. E. & Hoagland, C. L. (1942). Bact. Rev. 6, 79.CrossRefGoogle Scholar
Stone, J. D. & Burnet, F. M. (1946). Aust. J. Exp. Biol. Med. Sci. 24, 9.CrossRefGoogle Scholar
Watson, D. W., Cromartie, W. J., Bloom, W. L., Kegeles, G. & Heckley, R. J. (1946). J. Infect. Dis. 80, 28.CrossRefGoogle Scholar
Wishart, F. O. & Craigie, J. (1936). J. Exp. Med. 64, 831.CrossRefGoogle Scholar