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Studies on the LS antigen of vaccinia virus

Published online by Cambridge University Press:  15 May 2009

C. J. M. Rondle  and
J. D. Williamson
C. J. M. Rondle
Affiliation:
Department of Bacteriology and Immunology, London School of Hygiene and Tropical Medicine
J. D. Williamson
Affiliation:
Virology Department, Wright–Fleming Institute of Microbiology, St Mary's Hospital Medical School, London
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A material (LS) analogous to that described by Craigie & Wishart (1936a) has been detected in extracts of vaccinia-infected rabbit dermis. Gel-diffusion tests on this material support the view that it is a single substance possessing two distinct serologically reactive sites. The tests also show that under some conditions rabbit LS may give two lines of precipitation against suitable antisera and that this is not due usually to the dissociation of LS into discrete L and S substances.

A study of vaccinia-infected sheep dermis failed to give evidence of a single LS substance. Instead two chemically different substances were isolated, one having the serological specificity of L, the other that of S. The physical, chemical and quantitative serological properties of sheep S were such that it could not have arisen by degradation of an LS material similar to that found in vaccinia-infected rabbit dermis. On this evidence it is suggested that vaccinia virus is not necessarily synthesized in the same way in different host tissues.

Type
Original Articles
Information
Journal of Hygiene , Volume 66 , Issue 3 , September 1968 , pp. 415 - 426
Copyright
Copyright © Cambridge University Press 1968

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