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The specific detection of foot-and-mouth disease virus whole particle antigen (140S) by enzyme labelled immunosorbent assay

  • E. M. E. Abu Elzein (a1) and J. R. Crowther (a1)

Summary

A solid-phase micro-enzyme-labelled immunosorbent assay (ELISA) using guinea pig antiserum against purified (140S) inactivated foot-and-mouth disease (FMD) virus has been usedin a sandwich technique to specifically measure 140S virus in the presence of 12S material.

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References

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Abu Elzein, E. M. E. & Crowther, J. R. (1978). Enzyme-labelled immunosorbent assay techniques in foot-and-mouth disease virus research. Journal of Hygiene 80, 391–9.
Avrameas, S. (1969). Coupling of enzymes to proteins with gluteraldehyde. Immunochemistry 6, 4352.
Bachrach, H. L., Trautman, R. & Breese, S. S. (1964) Chemical and physical properties of virtually pure foot-and-mouth disease virus. American Journal of Veterinary Research 25, 333–42.
Brooksby, J. B. (1952). The technique of complement fixation in foot-and-mouth diesase research. Agricultural Research Council Report Series, no. 12 London: H.M.S.O.
Brown, F. & Cartwright, B. (1963). Purification of radioactive foot-and-mouth disease virus. Nature, London 199, 1168–70.
Capstick, P. B., Garland, A. J. M., Chapman, W. G. & Masters, R. C. (1967). Factors affecting the production of foot-and-mouth disease virus in deep suspension culture of BHK 21 clone 13 cells. Journal of Hygiene 65, 273–80.
Crowther, J. R. & Abu Elzein, E. M. E. (1979). Detection and quantification of foot-and-mouth disease virus by enzyme labelled immunosorbent assay technique. Journal of General Virology 42, 597602.
Garland, A. J. M., Mowat, G. N. & Fletton, B. (1977). An evaluation of some methods of assay of foot-and-mouth disease antigen for vaccines. International Symposium on Foot-and-Mouth Disease, Lyon 1976. Developments in Biological Standardisation 35 323–32.
Voller, A., Bidwell, D. & Bartlett, A. (1976) Microplate enzyme immunoassays for the immunodiagnosis of virus infections. Manual of Clinical Immunology 69 506–12. American Society for Microbiology, Washington.

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