Hostname: page-component-76fb5796d-vvkck Total loading time: 0 Render date: 2024-04-26T05:33:32.576Z Has data issue: false hasContentIssue false
  • English
  • Français

Some observations on the reaction between horse serum and a pool of rabbit antihorse serum: I. The relative inhibitory effect of excess antigen and excess antiserum

Published online by Cambridge University Press:  15 May 2009

G. R. E. Naylor
G. R. E. Naylor
Affiliation:
From the Department of Pathology, Cambridge
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. The participation times of a series of mixtures of horse serum and a pool of rabbit antihorse serum have been determined. The results have been presented in a table and isochrones drawn. Four distinct α procedure optima were found, suggesting that there may be at least four different antigen-antibody reactions concerned when horse serum reacts with a rabbit antihorse serum.

2. The reaction between horse serum crystalbumin and the same pooled rabbit antihorse serum has been investigated, and recorded-similarly.

3. Inhibition of precipitation was found to be more marked in the region of antigen excess than in the region of antiserum excess. Evidence is presented in support of the view that the occurrence of multiple zones may obscure this observation.

4. The results are compatible with the view that the second stage of the precipitation reaction is specific.

5. The isochrones correspond with Boyd's R type.

Type
Research Article
Information
Journal of Hygiene , Volume 46 , Issue 2 , July 1948 , pp. 129 - 135
Copyright
Copyright © Cambridge University Press 1948

References

REFERENCES

Boyd, W. C. (1941). Influence of character of antibody upon velocity of flocculation. J. exp. Med. 74, 369.CrossRefGoogle ScholarPubMed
Cruickshank, J. C. (1946). Quoted in Topley and Wilson's Principles of Bacteriology and Immunity, 3rd ed. p. 203. Edited by Wilson, G. S. and Miles, A. A.Google Scholar
Dean, H. R. & Webb, R. A. (1926). The influence of optimal proportions of antigen and antibody in the serum precipitation reaction. J. Path. Bact. 29, 473.CrossRefGoogle Scholar
Duncan, J. T. (1932). The use of equivalent proportions of antigen and serum in absorption of precipitin. Brit. J. exp. Path. 13, 489.Google Scholar
Goldsworthy, N. E. (1928). The occurrence of multiple zones in the serum precipitation reaction. J. Path. Bact. 31, 525.CrossRefGoogle Scholar
Goldsworthy, N. E. & Rudd, G. V. (1935). Complexity of antigens in relation to zones in the precipitation reaction. J. Path. Bact. 40, 169.CrossRefGoogle Scholar
Hewitt, L. F. (1938). Preparation and properties of a globulin present in the albumin fraction of serum. Biochem. J. 32, 26.CrossRefGoogle ScholarPubMed
Smith, W. (1932). The titration of antipneumococcus serum. J. Path. Bact. 35, 509.CrossRefGoogle Scholar
Taylor, G. L. (1931). The results of some quantitative experiments on the serum precipitation reaction. J. Hyg., Camb., 31, 56.CrossRefGoogle ScholarPubMed
Taylor, G. L. (1933). The dissimilarity of the results of precipitin titrations performed with a constant amount of antiserum and with a constant amount of antigen. J. Hyg., Camb., 33, 12.CrossRefGoogle ScholarPubMed
Taylor, G. L. & Adair, Muriel E. (1935). The precipitation reaction. Experiments on multiple zones. J. Hyg., Camb., 35, 169.CrossRefGoogle ScholarPubMed