Hostname: page-component-848d4c4894-nmvwc Total loading time: 0 Render date: 2024-06-27T16:34:44.362Z Has data issue: false hasContentIssue false
  • English
  • Français

Sodium Diethyldithiocarbamate and Oxine in the Differentiation of Brucella Species

Published online by Cambridge University Press:  15 May 2009

J. C. Cruickshank
J. C. Cruickshank
Affiliation:
London School of Hygiene and Tropical Medicine
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The effect of two chelating agents, sodium diethyldithiocarbamate and 8-hydroxy-quinoline (oxine), on 70 strains of brucellae (24 Br. abortus, 22 Br. melitensis and 28 Br. suis) has been examined.

The pattern of growth and inhibition of the three species as it appears in the carbamate test described by Renoux is of some value in the differentiation of species. Br. suis is more resistant to carbamate than the other two species.

The three species also differ in their sensitivity to oxine, and strains of Br. suis from various sources were consistently more resistant than the other species. This observation may be of use in the identification of Br. suis.

My thanks are due to Prof. Adrian Albert and Dr J. H. Marshall for helpful discussion of the chemical aspects of this work, and to Mr B. Madge, A.I.M.L.T., for technical assistance.

Type
Research Article
Information
Journal of Hygiene , Volume 53 , Issue 3 , September 1955 , pp. 305 - 312
Copyright
Copyright © Cambridge University Press 1955

References

Albert, A., Gibson, M. I. & Rubbo, S. D. (1953). The influence of chemical constitution on anti-bacterial activity. Part VI. The bactericidal action of 8-hydroxyquinoline (Oxine). Brit. J. exp. Path. 34, 119.Google Scholar
Albert, A., Rubbo, S. D., Goldacre, R. & Balfour, B. (1947). The influence of chemical constitution on anti-bacterial activity. Part III. A study of 8-hydroxyquinoline (Oxine) and related compounds. Brit. J. exp. Path. 28, 69.Google Scholar
Gale, E. F. & Mitchell, P. D. (1949). The assimilation of amino acids by bacteria. 8. Trace metals in glutamic acid assimilation and their inactivation by 8-hydroxyquinoline. J. gen. Microbiol. 3, 369.CrossRefGoogle ScholarPubMed
Hewitt, L. F. (1950). Oxidation-reduction Potentials in Bacteriology and Biochemistry, 6th ed. Edinburgh: E. and S. Livingstone.CrossRefGoogle Scholar
Pickett, M. J., Nelson, E. L. & Liberman, J. D. (1953). Speciation within the genus Brucella. II. Evaluation of differential dye, biochemical and serological tests. J. Bact. 66, 210.CrossRefGoogle ScholarPubMed
Renoux, G. (1952). Une nouvelle méthode de différentiation des variétés de Brucella. Action du diethyl-dithiocarbamate de soude (DEDTC). Ann. Inst. Pasteur, 82, 556.Google Scholar
Rubbo, S. D., Albert, A. & Gibson, M. I. (1950). The influence of chemical constitution on anti-bacterial activity. Part V. The anti-bacterial action of 8-hydroxyquinoline (Oxine). Brit. J. exp. Path. 31, 425.Google Scholar
Szybalski, W. & Bryson, V. (1952). Genetic studies on microbial cross resistance to toxic agents. I. Cross resistance of Escherichia coli to fifteen antibiotics. J. Bact. 64, 489.CrossRefGoogle ScholarPubMed
Tuttle, C. D. & Huddleson, I. F. (1934). Oxidation-reduction studies of growth differentiation of species of Brucella. J. infect. Dis. 54, 259.CrossRefGoogle Scholar