Skip to main content Accessibility help
×
Home

A slowly sedimenting infectious component of Rift Valley fever virus

  • A. Polson (a1) and Julia Levitt (a1)

Extract

If Rift Valley fever virus (RVF), of which the main component has a sedimentation constant (S20) of about 450, is centrifuged under conditions adequate to secli ment MEF1 poliomyelitis virus (S20 = 156) completely, about 1 % of the original infective particles remain in the supernatant fluid. This slowly sedimenting (S10W) fraction was shown by centrifugation to contain infective components ranging in sedimentation constant from 4 to 19 Svedberg units. Density gradient centrifu gation showed that the densities of these particles varied from 1 to < 1 g./cm. The components were neutralized by RVF immune serum but were not affected by ribonuclease or deoxyribonuclease. In gel diffusion-filtration experiments the S10W virus behaved as substances having very low diffusion constants. Its filtration end-point using graded collodion membranes, is approximately two to three times higher than the ‘whole’ virus. It is suggested that particles comprising the S10W virus fraction contain lipoid material and may be filamentous.

The authors wish to express their gratitude to Prof. A. Kipps and R. A. Alexander for their continued interest in this work and to Dr T. H. Mead for many helpful suggestions during preparation of the manuscript.

This investigation was supported in part by a Public Health Service research grant Al 04044–02 from the National Institute of Health, Bethesda, U.S.A.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      A slowly sedimenting infectious component of Rift Valley fever virus
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      A slowly sedimenting infectious component of Rift Valley fever virus
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      A slowly sedimenting infectious component of Rift Valley fever virus
      Available formats
      ×

Copyright

References

Hide All
Andrewes, C. H. & Horstmann, D. M. (1949). The susceptibility of viruses to ethyl ether. J. yen. Microbiol. 3, 390–7.
Casals, J. & Olitsky, P. K. (1950). A complement fixation test for poliomyelitis virus. Proc. Soc. exp. Biol., N.Y., 75, 315–18.
Casals, J., Olitsky, P. K. & Anslow, R. O. (1951). A specific complement fixation test for infection with poliomyelitis virus. J. exp. Med. 94, 123–37.
Cheng, P. Y. (1961). Some physical properties of hemagglutinating and complement fixing particles of Semliki Forest virus. Virology, 14, 132–40.
Crawford, L. V. (1960). A study of Rous sarcoma virus by density gradient centrifugation. Virology, 12, 143–53.
Gessler, A. E., Bender, C. E. & Parkinson, M. C. (1956). Animal viruses isolated by fluorocarbon emulsification. Trans. N.Y. Acad. Sci. 18, 707.
Hampton, J. W. F. (1958). A physicochemical examination of West Nile virus. Thesis, University of the Witwatersrand.
Herriott, R. M. (1961). Infectious nucleic acids, a new dimension in virology. Science, 134, 256–60.
Joubert, F. J. (1954). Haemocyanin of the crawfish (Jasus lalandii). Biochim. biophys. acta, 14, 127–35.
Kahier, H., Bryan, W. R., Lloyd, B. J. & Maloney, J. B. (1954). The density of the Rous sarcoma virus in sucrose solutions. J. nat. Cancer Inst. 15, 331–6.
Kipps, A., Turner, G. S. & Polson, A. (1961). Some properties of a cytopathogenic bovine orphan virus (van den Ende strain). J. gen. Microbiol. 26, 405–13.
Mayer, M. M., Osler, A. G., Bier, O. G. & Heidelberger, M. (1946). The activating effect of magnesium and other cations on the hemolytic function of complement. J. exp. Med. 84, 535–48.
Naudé, W. Du T., Madsen, T. I. & Polson, A. (1954). Different-sized infective particles of Rift Valley fever virus. Nature, Lond., 173, 1051–2.
Polson, A. (1954). Particle size of yellow fever virus. Proc. Soc. exp. Biol., N.Y., 85, 613–15.
Polson, A. (1956). Calculation of molecular weights from viscosity and diffusion data. Biochim. data. biophys. acta, 21, 185–7.
Polson, A. (1961 a). Fractionation of protein mixtures on columns of granulated agar. Biochim, biophys. acta, 50, 565–7.
Polson, A. (1961 b). A simplified fraction collector for gradient elution chromatography. J. Chromatog. 5, 116–20.
Polson, A. & Deeks, D. (1960). The diffusion constants of rabbit, guinea pig and rat anti-bodies to a single common antigen. Biochim. biophys. acta, 39, 208–11.
Polson, A. & Levitt, J. (1963). Density determination in a preformed gradient of caesium chloride. Biochim. biophys. acta, 75, 8895.
Polson, A. & Linder, A. M. (1953). The determination of the sedimentation constants of proteins and viruses with the help of the Spinco preparative ultracentrifuge. Biochim. biophys. acta, 11, 199208.
Polson, A. & Madsen, T. (1954). Particle size distribution of African homesickness virus. Biochim. biophys. acta, 14, 366–73.
Polson, A. & Selzer, G. (1952). Particle size of the MEF1 Lansing strain of poliomyelitis virus. Proc. Soc. exp. Biol., N.Y., 81, 218–22.
Polson, A. & van Regenmortel, M. H. V. (1961). A new method for determination of sedimentation constants of viruses. Virology, 15, 397403.
Reed, L. J. & Muench, A. (1938). A simple method for estimating fifty per cent endpoints. Amer. J. Hyg. 27, 493–7.
Selzer, C. & Polson, A. (1954). Dispersion analyses of the MEF1 strain of poliomyelitis virus. Biochim. biophys. acta, 15, 251–7.
Selzer, G., Sacks, M. & van den Ende, M. (1952). Adaptation and multiplication rate of the MEF strain of poliomyelitis virus in newborn mice. S. Afr. med. J. 26, 201–4.
Svedberg, T. & Pedersen, K. O. (1940). The Ultracentrifuge. Oxford: Clarendon Press.

A slowly sedimenting infectious component of Rift Valley fever virus

  • A. Polson (a1) and Julia Levitt (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed