Hostname: page-component-7bb8b95d7b-495rp Total loading time: 0 Render date: 2024-09-11T12:33:15.607Z Has data issue: false hasContentIssue false
  • English
  • Français

Salmonella isolation with Rappaport's medium after pre-enrichment in buffered peptone water using a series of inoculum ratios

Published online by Cambridge University Press:  15 May 2009

R. W. S. Harvey  and
T. H. Price
R. W. S. Harvey
Affiliation:
Regional Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff CF4 4XW
T. H. Price
Affiliation:
Regional Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff CF4 4XW
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The ability of malachite green/magnesium chloride broth (Rappaport's medium) to isolate salmonellas from 25 ml quantities of sewage-polluted natural water was investigated. Samples were first pre-enriched in buffered peptone water and varying volumes of inoculum from the pre-enrichment culture were inoculated into Rappaport's broth. Inoculum ratios in the range 1:2000 to 1:10 were examined. The inoculum ratio denotes the ratio of the volume of inoculum to the volume of fluid medium into which it is introduced. Optimum results were obtained with the 1:2000 ratio, although the salmonella isolation rate was only slightly less with the 1:500 and 1:100 ratios. The 1:2000 inoculum ratio was obtained with a graduated loop holding approximately 0·005 ml of fluid. Use of a loop for inoculation has advantages in speed of performance and safety of manipulation.

Type
Research Article
Information
Journal of Hygiene , Volume 85 , Issue 1 , August 1980 , pp. 125 - 128
Copyright
Copyright © Cambridge University Press 1980

References

Anon (1975). Meat and Meat Products – Detection of Salmonellae (reference method). International Standard ISO 3565-1975 (E). Geneva: International Organization for Standardization.Google Scholar
Collard, P. & Unwin, M. (1958). A trial of Rappaport's medium. Journal of Clinical Pathology 11, 426.CrossRefGoogle ScholarPubMed
Edel, W. & Kampelmacher, E. W. (1973). Comparative studies on isolation methods of ‘sub-lethally’ injured salmonellae in nine European laboratories. Bulletin of the World Health Organization 48, 167.Google Scholar
Harvey, R. W. S. (1956). Choice of a selective medium for the routine isolation of members of the salmonella group. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 15, 118.Google ScholarPubMed
Harvey, R. W. S. & Price, T. H. (1974). Isolation of Salmonellas. Public Health Laboratory Service Monograph Series 8. London: HMSO.Google Scholar
Harvey, R. W. S. & Price, T. H. (1977). Observations on pre-enrichment for isolating salmonellas from sewage-polluted natural water using Muller–Kauffmann tetrathionate prepared with fresh and desiccated ox bile. Journal of Applied Bacteriology 43, 145.CrossRefGoogle ScholarPubMed
Harvey, R. W. S., Price, T. H. & Crone, P. B. (1975). Quality control tests of two salmonella enrichment media using different inocula. Journal of Hygiene 74, 375.CrossRefGoogle ScholarPubMed
Harvey, R. W. S., Price, T. H. & Xirouchaki, E. (1979). Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique. Journal of Hygiene 83, 451.CrossRefGoogle ScholarPubMed
Jameson, J. E. (1961). A study of tetrathionate enrichment techniques, with particular reference to two new tetrathionate modifications used in isolating salmonellae from sewage swabs. Journal of Hygiene 59, 1.CrossRefGoogle Scholar
Jameson, J. E. (1963). A note on the isolation of salmonellae. Journal of Applied Bacteriology 26, 112.CrossRefGoogle Scholar
Loeffler, F. (1906). Der Kulturelle Nachweiss der Typhusbazillen in Faeces, Erde und Wasser mit Hilfe des Malachitgrüns. Deutsche Medizinische Wochenschrift 32, 289.CrossRefGoogle Scholar
Rappaport, F., Konforti, N. & Navon, B. (1956). A new enrichment medium for certain salmonellae. Journal of Clinical Pathology 9, 261.CrossRefGoogle Scholar
Vassiliadis, P., Trichopoulos, D., Papadakis, J. & Politi, G. (1970). Salmonella isolations in abattoirs in Greece. Journal of Hygiene 68, 601.Google ScholarPubMed