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The release of influenza virus from the infected cell

Published online by Cambridge University Press:  15 May 2009

L. Hoyle
L. Hoyle
Affiliation:
Public Health Laboratory, General Hospital, Northampton
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1. When detached portions of the chorioallantoic membranes of normal fertile eggs are examined in the dark-field microscope spherical and tubular cytoplasmic protrusions are seen to develop from the margins of the cells and to become detached from them. This is probably a normal physiological phenomenon which occurs to some extent in the intact egg.

2. Similar cell protrusions occur from chorioallantoic membranes of eggs infected with influenza virus. The phenomenon appears to be more pronounced in the infected egg than in the normal egg and in particular tubular cytoplasmic protrusions are much more frequent in the infected egg than in the normal.

3. Tubular cytoplasmic protrusions may fragment into small spherical particles or may contract into filaments. All types of cell protrusion become detached from the cells and undergo contraction in size probably as a result of loss of water. The infective elementary bodies and filaments present in the allantoic fluid of eggs infected with influenza virus are derived from the cells in this way.

4. It is concluded that the infective particle of the influenza virus is a fragment of the cell cytoplasm and consists of a closely aggregated mass of virus protein enclosed in cell membrane.

The author is indebted to Prof. W. T. Astbury and Dr R. Reed for the electron photomicrographs reproduced in this paper.

Type
Research Article
Information
Journal of Hygiene , Volume 52 , Issue 2 , June 1954 , pp. 180 - 188
Copyright
Copyright © Cambridge University Press 1954

References

REFERENCES

Burnet, F. M. & Lind, P. E. (1951 a). A genetic approach to variation in influenza viruses. 3. Recombination of characters of influenza virus strains used in mixed infections. J. gen. Microbiol. 5, 59.CrossRefGoogle Scholar
Burnet, F. M. & Lind, P. E. (1951 b). A genetic approach to variation in influenza viruses. 4. Recombination of characters between the influenza virus A strain NWS and strains of different serological subtypes. J. gen. Microbiol. 5, 67.CrossRefGoogle Scholar
Chu, C. M., Dawson, I. M. & Elford, W. J. (1949). Filamentous forms associated with newly isolated influenza virus. Lancet, 1, 602.CrossRefGoogle ScholarPubMed
Eddy, B. E. & Wyckoff, R. W. G. (1950). Influenza virus in sectioned tissues. Proc. Soc. exp. Biol., N.Y., 75, 290.CrossRefGoogle ScholarPubMed
Heinmetz, F. (1948). Studies with the electron microscope on the interaction of red cells and influenza virus. J. Bact. 55, 823.CrossRefGoogle Scholar
Henle, W. & Henle, G. (1949). Studies on host-virus interactions in the chick embryo-influenza virus system. III. Development of infectivity haemagglutination, and complement fixation activities in the first infectious cycle. J. exp. Med. 90, 23.CrossRefGoogle Scholar
Hoyle, L. (1948). The growth cycle of influenza virus A. A study of the relations between virus, soluble antigen and host cell in fertile eggs inoculated with influenza virus. Brit. J. exp. Path. 29, 390.Google ScholarPubMed
Hoyle, L. (1950). The multiplication of influenza viruses in the fertile egg. J. Hyg., Camb., 48, 277.CrossRefGoogle ScholarPubMed
Hoyle, L. (1953). The multiplication of the influenza virus considered in relation to the general problem of biological multiplication. The Nature of Virus Multiplication, p. 255. Cambridge University Press.Google Scholar
Hoyle, L., Reed, R. & Astbury, W. T. (1953). Electron microscope studies of the structure of influenza virus. Nature, Lond., 171, 256.CrossRefGoogle ScholarPubMed
Mosley, V. M. & Wyckoff, R. W. G. (1946). Electron microscopy of the virus of influenza. Nature, Lond., 157, 263.CrossRefGoogle Scholar
Murphy, J. S. & Bang, F. B. (1952). Observations with the electron microscope on cells of the chick chorioallantoic membrane infected with influenza virus. J. exp. Med. 95, 259.CrossRefGoogle ScholarPubMed
Murphy, J. S., Karzon, D. T. & Bang, F. B. (1950). Studies of influenza A (PR 8) infected tissue cultures by electron microscopy. Proc. Soc. exp. Biol., N.Y., 73, 596.CrossRefGoogle Scholar
Wyckoff, R. W. G. (1951). Electron microscopy of chick embryo membrane infected with PR 8 influenza. Nature, Lond., 168, 651.CrossRefGoogle ScholarPubMed
Wyckoff, R. W. G. (1953). Formation of the particles of influenza virus. J. Immunol. 70, 187.CrossRefGoogle ScholarPubMed
Zollinger, H. U. (1948). Cytologic studies with the phase microscope. The formation of ‘blisters’ on cells in suspension (potocytosis) with observations on the nature of the cellular membrane. Amer. J. Path. 24, 545.Google Scholar