Hostname: page-component-76fb5796d-9pm4c Total loading time: 0 Render date: 2024-04-26T05:13:27.531Z Has data issue: false hasContentIssue false
  • English
  • Français

The relative effectiveness of commonly used disinfectants in inactivation of echovirus 11

Published online by Cambridge University Press:  15 May 2009

Murray Drulak ,
Alfred M. Wallbank  and
Ingrid Lebtag
Murray Drulak
Affiliation:
The Department of Medical Microbiology and Infectious Diseases, University of Manitoba Medical College, Winnipeg, Manitoba, CanadaR3E 0W3
Alfred M. Wallbank
Affiliation:
The Department of Medical Microbiology and Infectious Diseases, University of Manitoba Medical College, Winnipeg, Manitoba, CanadaR3E 0W3
Ingrid Lebtag
Affiliation:
The Department of Medical Microbiology and Infectious Diseases, University of Manitoba Medical College, Winnipeg, Manitoba, CanadaR3E 0W3
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Echovirus 11 in the presence of fetal calf serum was exposed to six commonly used disinfectants for times of 10, 20 and 30 s. At the end of such exposure times, skim milk neutralized disinfectant activity and residual virus was assayed using the plaque technique. The six disinfectants studied were Javex, sodium hydroxide, ethanol, Wescodyne, One Stroke Ves-Phene, and Sonacide. Although 0·25% (w/v) sodium hydroxide and 95% (v/v) ethanol were equally virucidal and significantly more so than the other four disinfectants, causing 106 reduction in 20 s, they may not be practical to use in many instances. Javex at a dilution of 1/50 (1200 parts/106 chlorine) proved to be virucidal causing 103·5 reduction of echovirus 11 in 30 s. Wescodyne (1/50) and undiluted Sonacide were relatively ineffective causing 10 reduction or less of echovirus 11 in 30 s. One Stroke Ves-Phene (1/50) was ineffective causing no significant inactivation in 30 s.

Type
Research Article
Information
Journal of Hygiene , Volume 81 , Issue 1 , August 1978 , pp. 77 - 87
Copyright
Copyright © Cambridge University Press 1978

References

REFERENCES

Association Of Official Agricultural Chemists (1965). Official Methods of Analysis 10th ed. Association of Official Agricultural Chemists, Washington, D.C.Google Scholar
Barile, M. F. (1973). Mycoplasmal Contamination of Cell Cultures: Mycoplasma-virus-Cell Culture Interactions Contamination in Tissue Culture, ch. 6. New York and London: Academic Press Inc.Google Scholar
Barron, A. L. C., Olshevsky, C. & Cohen, M. M. (1970). Characteristics of the BGM line of cells from African green monkey kidney. Archiv für die gesamte Virusforschung 32, 389–92.CrossRefGoogle ScholarPubMed
Bergen, T. & Lystad, A. (1972). Evaluation of disinfectant inactivators. Acta pathologica et microbiologica scandinavica, Section B, 80, 507–10.Google Scholar
Darling, D. R., Berg, G. & Berman, D. (1974). BGM; A continuous cell line more sensitive than primary Rhesus and African green kidney cells for the recovery of viruses from water. Health Laboratory Science 11, 275–82.Google Scholar
Dulbecco, R. & Vogt, M. (1954). Plaque formation and isolation of pure lines with poliomyelitis viruses. Journal of Experimental Medicine 99, 167–82.CrossRefGoogle ScholarPubMed
Gaustad, J. W., Mcduef, C. R. & Hatcher, H. J. (1974). Test method for the evaluation of virucidal efficacy of three common liquid surface disinfectants on a simulated environmental surface. Applied Microbiology 28, 748–52.CrossRefGoogle ScholarPubMed
Groupé, V., Engle, C. G., Gaffney, P. E. & Manaeer, R. A. (1955). Virucidal activity of representative antiinfective agents against influenza A and vaccinia viruses. Applied Microbiology 3, 333–6.CrossRefGoogle ScholarPubMed
Lorenz, D. E. & Jann, G. J. (1964). Use-dilution test and Newcastle disease virus. Applied Microbiology 12, 24–6.CrossRefGoogle ScholarPubMed
Mcduff, C. R. & Gaustad, J. W. (1976). Test method for determination of virucidal efficacy of liquid surface disinfectants. Journal of the Association of Official Analytical Chemists 59, 1150–55.Google ScholarPubMed
Rand, M. C., Greenberg, A. E. & Tarros, M. J. (1975). Standard Methods for the Examination of Water and Wastewater, 14th edn, pp. 322–5. American Health Association, Washington, D.C.Google Scholar
Steel, R. & Torrie, J. (1960). Principles and Procedures of Statistics, ch. 10, p. 187. New York: McGraw and Hill Co.Google Scholar
West Chemical Products Inc. Wescodyne. Detergent-Germicide for hospital use containing ‘Tamed Iodine’, West Chemical Products Inc. 42–16 West Street, Long Island City, N.Y. 11101.Google Scholar
Wright, H. S. (1970). Test method for determining the virucidal activity of disinfectants against vesicular stomatitis virus. Applied Microbiology 19, 92–5.CrossRefGoogle Scholar