Skip to main content Accessibility help
×
Home

Prospective study of verocytotoxin–producing, enteroaggregative and diffusely adherent Escherichia coli in different diarrhoeal states

  • M. G. Brook (a1), H. R. Smith (a2), B. A. Bannister (a1), M. McConnell (a2), H. Chart (a2), S. M. Scotland (a2), A. Sawyer (a3), M. Smith (a3) and B. Rowe (a2)...

Summary

One hundred and eighty–one stool specimens from patients with various types of diarrhoea (135 patients) or from non-diarrhoeal controls (23 acute medical patients, 23 inflammatory bowel disease in remission) were investigated using a colony–blot DNA hybridization assay for the presence of Verocytotoxin–producing (VTEC), enteroaggregative (EAggEC) and diffusely adherent (DAEC) Escherichia coli. Twelve patients had probe–positive EAggEC in the stool and 8 of these had diarrhoea, 6 following recent travel. Eight patients had DAEC, 7 of whom had travellers diarrhoea. Six of 10 (60%) travellers with gastroenteritis, but without a recognized enteric pathogen, were positive for EAggEC (4) or DAEC (2). Five of 10 (50%) travellers with gastroenteritis related to a recognized enteric pathogen also had DAEC identified in their stool. Of the 23 acute medical control patients 11 had been abroad, 4 of these were immigrants and had EAggEC. VTEC were not found and, with one exception, immunoassays for antibodies to E. coli O 157 and O 2 lipopolysaccharides were negative.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Prospective study of verocytotoxin–producing, enteroaggregative and diffusely adherent Escherichia coli in different diarrhoeal states
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Prospective study of verocytotoxin–producing, enteroaggregative and diffusely adherent Escherichia coli in different diarrhoeal states
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Prospective study of verocytotoxin–producing, enteroaggregative and diffusely adherent Escherichia coli in different diarrhoeal states
      Available formats
      ×

Copyright

Corresponding author

*Correspondence and reprint requests: Department of Infectious and Tropical Diseases, Coppetts Wood Unit, Royal Free Hospital NHS Trust, Coppetts Road, London N10 1JN.

References

Hide All
Levine, MM. Escherichia coli that cause diarrhoea: enterotoxigenic, enteropathogenic. enteroinvasive, enterohemorrhagic and enteroadherent. J Infect Dis 1987; 155: 377–89.
Mathewson, JJ, Johnson, PP, Dupont, HL et al. , A newly recognised cause of travelers' diarrhea: enteroadherent Escherichia coli. J Infect Dis 1985; 151: 471–5.
Cravioto, A, Tello, A, Navarro, A et al. , Association of Escherichia coli HEp-2 adherence patterns with type and patterns of diarrhea. Lancet 1991; 337: 262–4.
Giron, JA, Jones, T, Millan-Velasco, F et al. , Diffuse adhering Escherichia coli (DAEC) as a putative cause of diarrhoea in Mayan children in Mexico. J Infect Dis 1991; 163: 507–13.
Bhan, MK, Raj, P, Levine, MM et al. , Enteroaggregative Escherichia coli associated with persistent diarrhea in a cohort of rural children in India. J Infect Dis 1989; 159: 1061–3.
Ostroff, SM, Kobayashi, JM, Lewis, JH. Infections with Escherichia coli O 157: H7 in Washington State. JAMA 1988; 262: 355–9.
Karmali, MA. Infection by verocytotoxin producing Escherichia coli. Clin Microbiol Rev 1989; 2: 1538.
Chart, H, Smith, HR, Scotland, SM, Rowe, B, Milford, DW, Taylor, CM. Serological identification of Escherichia coli O 157: H7 infection in haemolytic uraemic syndrome. Lancet 1991: 337: 138–40.
von Wullfen, H, Russmann, H, Karch, H et al. , Verocytotoxin-producing Escherichia coli O 2: H5 isolated from patients with ulcerative colitis. Lancet 1989; i: 1449–50.
Willshaw, GA, Scotland, SM, Smith, HR, Rowe, B. Properties of Verocytotoxin-producing Escherichia coli of human origin belonging to serogroups other than O 157. J Infect Dis 1992; 166: 797802.
Baudry, B, Savarino, SJ, Vial, P, Kaper, JB, Levine, MM. A sensitive and specific DNA probe to identify enteroaggregative Escherichia coli, a recently discovered diarrhoeal pathogen. J Infect Dis 1990; 161: 1249–51.
Scotland, SM, Willshaw, GA, Smith, HR, Gross, RJ, Rowe, B. Adhesion to cells in culture and plasmid profiles of enteropathogenic Escherichia coli isolated from outbreaks and sporadic cases of infant diarrhoea. J Infect 1989; 19: 237–49.
Chart, H, Scotland, SM, Rowe, B. Serum antibodies to Escherichia coli serotype O 157 in patients with haemolytic uraemic syndrome. J Clin Microbiol 1989; 27: 285–90.
Walker, CW, Upson, R, Warren, RE. Haemorrhagic colitis: detection of verotoxin producing Escherichia coli in a clinical microbiological laboratory. J Clin Pathol 1988; 41: 80–4.
Tarr, PI, Neill, MA, Clausen, CR et al. , Escherichia coli O 157 and the hemolytic uremic syndrome: importance of early culture in establishing the etiology. J Infect Dis 1990; 162: 553–6.
Karch, H, Meyer, T, Russmann, H, Heesemann, J. Frequent loss of shiga-like toxin genes in clinical isolates of Escherichia coli on subcultivation. Infect Immun 1992; 60: 3464–7.
Nalin, DR. Enteric bacterial infections. Introduction and general principles. In: Strickland, GT. ed., Hunter's tropical medicine. London: WB Saunders, 1984: 270–9.
Tacket, CO, Moseley, SL, Kay, B et al. , Challenge studies in volunteers using Escherichia coli strains with diffuse adherence to HEp-2 cells. J Infect Dis 1990; 162: 550–2.

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed