Hostname: page-component-76fb5796d-22dnz Total loading time: 0 Render date: 2024-04-25T08:58:02.197Z Has data issue: false hasContentIssue false
  • English
  • Français

The production of monoclonal antibodies to rubella haemagglutinin and their use in antibody-capture assays for rubella-specific IgM

Published online by Cambridge University Press:  25 March 2010

R. S. Tedder ,
J. L. Yao  and
M. J. Anderson
R. S. Tedder
Affiliation:
Department of Virology, Middlesex Hospital Medical School, Riding House Street, London W1P 7PP
J. L. Yao
Affiliation:
Department of Virology, Middlesex Hospital Medical School, Riding House Street, London W1P 7PP
M. J. Anderson
Affiliation:
Department of Medical Microbiology, King's College Hospital Medical School, Denmark Hill, London SE5 8RX
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Mice were immunized by three intraperitoneal and one intravenous injection of rubella haemagglutinin. Splenocytes from these mice were fused with the cells of a syngeneic myeloma cell line, and following culture for various periods of time, single-cell clones were derived by the technique of limiting dilution.

A total of 139 clones were derived from 13 parent hybrid cultures. To date, four of these cloned cultures have been propagated as ascitic tumours in mice. The preparation of IgG from ascitic fluid and labelling of this antibody with 125I is described. Results indicate that the use of labelled monoclonal antibodies as indicator reagents in solid-phase IgM antibody capture assays for the detection of rubella-specific IgM results in enhanced performance of these tests.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 2 , April 1982 , pp. 335 - 350
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

David, G. S., Present, W., Martinis, J., Wang, R., Bartholomew, R., Desmond, W. & Sevier, E. D. (1981). Monoclonal antibodies in the detection of hepatitis infection. Medical Laboratory Sciences 38, 341–348.Google ScholarPubMed
Diament, J. A. & Pepys, J. (1978). Immunosorbent separation of IgG and IgM for the radio-immunoassay of specific antibodies. In Affinity Chromatography (ed. Hoffmann-Ostenhoffer, et al. ), pp. 229–231. Oxford: Pergamom Press.CrossRefGoogle Scholar
Duermeyer, W., Wielaard, F. & Van der Veen, J. (1979). A new principle for the detection of specific IgM antibodies applied to an ELISA for hepatitis A. Journal of Medical Virology 4, 25–32.CrossRefGoogle Scholar
Gerhard, W., Yewdell, J., Frankel, M. E. & Webster, R. (1981). Antigenic structure of influenza virus haemagglutinin defined by hybridoma antibodies. Nature 290, 713–717.CrossRefGoogle ScholarPubMed
Gerlich, W. H. & Luer, W. (1979). Selective detection of IgM antibody against core antigen of the hepatitis B virus by a modified enzyme immunoassay. Journal of Medical Virology 4, 227–238.CrossRefGoogle Scholar
Goodall, A. H., Miescher, G., Meek, F. M., Janossy, G. & Thomas, H. C. (1981). Monoclonal antibodies in a solid phase radiometric assay for HBsAg. Medical Laboratory Sciences 38, 349–354.Google Scholar
Kangro, H. O., Pattison, J. R. & Heath, R. B. (1978). The detection of rubella-specific IgM antibodies by radioimmunoassay. British Journal of Experimental Pathology 59, 577–583.Google ScholarPubMed
Kohler, G. & Milstein, C. (1975). Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256, 495–497.CrossRefGoogle ScholarPubMed
Mortimer, P. P., Edwards, J. M. B., Porter, A. D., Tedder, R. S., Mace, J. E. & Hutchinson, A. (1981 a). Are many women immunized against rubella unnecessarily? Journal of Hygiene 87, 131–138.CrossRefGoogle ScholarPubMed
Mortimer, P. P., Tedder, R. S., Hambling, M. H., Shafi, M. S., Burkhardt, F. & Schilt, U. (1981 b). Antibody capture radioimmunoassay for anti-rubella IgM. Journal of Hygiene 86, 139–153.CrossRefGoogle ScholarPubMed
Pattison, J. R. & Mace, J. E. (1973). Rubella screening tests. Journal of Clinical Pathology 26, 161.CrossRefGoogle ScholarPubMed
Salacinski, P., Hope, J., McClean, C., Clement-Jones, V., Sykes, J., Price, J. & Lowry, P. J. (1979). A new simple method which allows theoretical incorporation of radioiodine into proteins and peptides without damage. Journal of Endocrinology 81, 131–137.Google ScholarPubMed
Schmidt, H., Deimling, U. von & Flehmig, B. (1980). Detection of IgM antibodies to cytomegalovirus (CMV) using an enzyme-labelled antigen (ELA). Journal of General Virology 50, 59–68.Google Scholar
Webster, R. G. & Berton, M. T. (1981). Analysis of antigenic drift in the haemagglutinin of influenza B virus with monoclonal antibodies. Journal of General Virology 54, 243–251.CrossRefGoogle ScholarPubMed