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The production of monoclonal antibodies to rubella haemagglutinin and their use in antibody-capture assays for rubella-specific IgM

  • R. S. Tedder (a1), J. L. Yao (a1) and M. J. Anderson (a2)

Summary

Mice were immunized by three intraperitoneal and one intravenous injection of rubella haemagglutinin. Splenocytes from these mice were fused with the cells of a syngeneic myeloma cell line, and following culture for various periods of time, single-cell clones were derived by the technique of limiting dilution.

A total of 139 clones were derived from 13 parent hybrid cultures. To date, four of these cloned cultures have been propagated as ascitic tumours in mice. The preparation of IgG from ascitic fluid and labelling of this antibody with 125I is described. Results indicate that the use of labelled monoclonal antibodies as indicator reagents in solid-phase IgM antibody capture assays for the detection of rubella-specific IgM results in enhanced performance of these tests.

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References

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David, G. S., Present, W., Martinis, J., Wang, R., Bartholomew, R., Desmond, W. & Sevier, E. D. (1981). Monoclonal antibodies in the detection of hepatitis infection. Medical Laboratory Sciences 38, 341348.
Diament, J. A. & Pepys, J. (1978). Immunosorbent separation of IgG and IgM for the radio-immunoassay of specific antibodies. In Affinity Chromatography (ed. Hoffmann-Ostenhoffer, et al. ), pp. 229231. Oxford: Pergamom Press.
Duermeyer, W., Wielaard, F. & Van der Veen, J. (1979). A new principle for the detection of specific IgM antibodies applied to an ELISA for hepatitis A. Journal of Medical Virology 4, 2532.
Gerhard, W., Yewdell, J., Frankel, M. E. & Webster, R. (1981). Antigenic structure of influenza virus haemagglutinin defined by hybridoma antibodies. Nature 290, 713717.
Gerlich, W. H. & Luer, W. (1979). Selective detection of IgM antibody against core antigen of the hepatitis B virus by a modified enzyme immunoassay. Journal of Medical Virology 4, 227238.
Goodall, A. H., Miescher, G., Meek, F. M., Janossy, G. & Thomas, H. C. (1981). Monoclonal antibodies in a solid phase radiometric assay for HBsAg. Medical Laboratory Sciences 38, 349354.
Kangro, H. O., Pattison, J. R. & Heath, R. B. (1978). The detection of rubella-specific IgM antibodies by radioimmunoassay. British Journal of Experimental Pathology 59, 577583.
Kohler, G. & Milstein, C. (1975). Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256, 495497.
Mortimer, P. P., Edwards, J. M. B., Porter, A. D., Tedder, R. S., Mace, J. E. & Hutchinson, A. (1981 a). Are many women immunized against rubella unnecessarily? Journal of Hygiene 87, 131138.
Mortimer, P. P., Tedder, R. S., Hambling, M. H., Shafi, M. S., Burkhardt, F. & Schilt, U. (1981 b). Antibody capture radioimmunoassay for anti-rubella IgM. Journal of Hygiene 86, 139153.
Pattison, J. R. & Mace, J. E. (1973). Rubella screening tests. Journal of Clinical Pathology 26, 161.
Salacinski, P., Hope, J., McClean, C., Clement-Jones, V., Sykes, J., Price, J. & Lowry, P. J. (1979). A new simple method which allows theoretical incorporation of radioiodine into proteins and peptides without damage. Journal of Endocrinology 81, 131137.
Schmidt, H., Deimling, U. von & Flehmig, B. (1980). Detection of IgM antibodies to cytomegalovirus (CMV) using an enzyme-labelled antigen (ELA). Journal of General Virology 50, 5968.
Webster, R. G. & Berton, M. T. (1981). Analysis of antigenic drift in the haemagglutinin of influenza B virus with monoclonal antibodies. Journal of General Virology 54, 243251.

The production of monoclonal antibodies to rubella haemagglutinin and their use in antibody-capture assays for rubella-specific IgM

  • R. S. Tedder (a1), J. L. Yao (a1) and M. J. Anderson (a2)

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