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The prevalence of Salmonella enteritidis and other Salmonella spp. among Canadian registered commercial layer flocks

Published online by Cambridge University Press:  15 May 2009

C. Poppe ,
R. J. Irwin ,
C. M. Forsberg ,
R. C. Clarke  and
J. Oggel
C. Poppe
Affiliation:
Health of Animals Laboratory, Agriculture Canada, 110 Stone Road West, Guelph, Ontario, CanadaN1G 3W4
R. J. Irwin
Affiliation:
Health of Animals Laboratory, Agriculture Canada, 110 Stone Road West, Guelph, Ontario, CanadaN1G 3W4
C. M. Forsberg
Affiliation:
Health of Animals Laboratory, Agriculture Canada, 110 Stone Road West, Guelph, Ontario, CanadaN1G 3W4
R. C. Clarke
Affiliation:
Health of Animals Laboratory, Agriculture Canada, 110 Stone Road West, Guelph, Ontario, CanadaN1G 3W4
J. Oggel
Affiliation:
Laboratory Services Division, Central Experimental Farm, Ottawa, Ontario, CanadaK1A 0C6
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Summary

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A survey was conducted to estimate the prevalence of Salmonella enteritidis and other salmonellas among Canadian commercial egg producing flocks. Environmental (faecal and eggbelt) samples from 152 of 295 (52·9%) randomly selected flocks were contaminated with salmonellas. Thirty-five different salmonella serovars were isolated. Eggbelt samples were more often contaminated with salmonellas than faecal samples (25·7 v. 10·1 %). The most prevalent serovars were S. heidelberg, S. infantis, S. hadar, and S. schwarzengrund; they were isolated from samples of 59/295 (20%), 18/295 (6·1%), 17/295 (5·8%), and 15/295 (5·1%) flocks, respectively. Feed samples of 21/295 (7·2%) flocks were contaminated with salmonellas. Salmonella enteritidis was isolated from the environmental samples of 8/295 (2·7%) flocks. Salmonella enteritidis phage type (PT) 8 was isolated from 5 flocks, PT 13a from 2 flocks, and PT 13 from 1 flock.

Type
Special Article
Information
Epidemiology & Infection , Volume 106 , Issue 2 , April 1991 , pp. 259 - 270
Copyright
Copyright © Cambridge University Press 1991

References

REFERENCES

1.Cowden, JM, Lynch, D, Joseph, CA et al. , Case-control study of infections with Salmonella enteritidis phage type 4 in England. Br Med J 1989; 299: 771–3.CrossRefGoogle ScholarPubMed
2.Frost, JA, Ward, LR, Rowe, B. Acquisition of a drug resistance plasmid converts Salmonella enteritidis phage type 4 to phage type 24. Epidemiol Infect 1989; 103: 243–8.CrossRefGoogle ScholarPubMed
3.Sharp, JCM. Salmonellosis and eggs. Br Med J 1989; 297: 1557–8.CrossRefGoogle Scholar
4.Perales, I, Audicana, A. Salmonella enteritidis and eggs. Lancet 1988; ii: 1133.CrossRefGoogle Scholar
5.Madden, JM. Increase in the numbers of cases of Salmonella enteritidis in the United States due to whole chicken eggs and the implications to food handlers. J Food Protect 1989; 52: 753.Google Scholar
6.Lior, H. Isolations of enteric pathogens from people in Canada. Safety Watch 1989; 14 (Fall): 3.Google Scholar
7.Hickman-Brenner, FW, Stubbs, AD, Farmer, JJ. Phage typing of Salmonella enteritidis in the United States. In: Morello, JA, Domer, JE, eds. Abstracts of the 90th annual meeting of the American Society for Microbiology. Washington: American Society for Microbiology, 1990: 403.Google Scholar
8.Miller, D. Summary of S. enteritidis, sources and phage types. In: Proceedings of the 39th annual meeting of the United States Animal Health Association. Richmond VA: Carter Printing, 1989: 555–6.Google Scholar
9.Cowden, JM, Chisholm, D, O'Mahony, M et al. , Two outbreaks of Salmonella enteritidis phage type 4 infection associated with the consumption of fresh shell-egg products. Epidemiol Infect 1989; 103: 47–52.CrossRefGoogle ScholarPubMed
10.Lee, LA. The public health impact of Salmonella enteritidis infection in the United States. In: Proceedings of the 39th annual meeting of the United States Animal Health Association. Richmond VA: Carter Printing, 1989: 548–9.Google Scholar
11.Lin, CF-Y, Morris, JG, Trump, D et al. , Investigation of an outbreak of Salmonella enteritidis gastroenteritis associated with consumption of eggs in a restaurant chain in Maryland. Am J Epidemiol 1988; 128: 839–44.CrossRefGoogle Scholar
12.St Louis, ME, Morse, DL, Potter, ME et al. , The emergence of grade A eggs as a major source of Salmonella enteritidis infections. J Am Med Assoc 1988; 259: 2103–7.CrossRefGoogle Scholar
13.Steinert, L, Virgil, D, Bellemore, E et al. , Update: Salmonella enteritidis infections and grade A shell eggs – United States, 1989. Morb Mort Weekly Rep 1990; 38: 877–80.Google Scholar
14.Snoeyenbos, GH, Smyser, FC, Van Roekel, H. Salmonella infections of the ovary and peritoneum of chickens. Avian Dis 1969; 13: 668–70.CrossRefGoogle ScholarPubMed
15.Eckroade, RJ, Benson, CE. Current information on S. enteritidis infected flocks and pathogenic characteristics. In: Proceedings of the 39th annual meeting of the United States Animal Health Association. Richmond VA: Carter Printing, 1989: 549–50.Google Scholar
16.Gast, RK, Beard, CW. Current information on SE: Experimentally infected chickens. In: Proceedings of the 39th annual meeting of the United States Animal Health Association. Richmond VA: Carter Printing, 1989: 550–1.Google Scholar
17.Hopper, SA, Mawer, S. Salmonella enteritidis in a commercial layer flock. Vet Rec 1988; 123: 351.CrossRefGoogle Scholar
18.Humphrey, TJ, Baskerville, A, Mawer, S, Rowe, B, Hopper, S. Salmonella enteritidis phage type 4 from the contents of intact eggs: a study involving naturally infected hens. Epidemiol Infect 1989; 103: 415–23.CrossRefGoogle ScholarPubMed
19.Kradel, DC, Benson, CE, Eckroade, RJ. The Salmonella enteritidis situation – preliminary epidemiological observations. 60th Northeastern Conference on Avian Diseases,Mystic Conn. 1988.Google Scholar
20.Snoeyenbos, GH, McKie, BA, Smyser, CF, Weston, CR. Progress in identifying and maintaining Salmonella-free commercial chicken breeding flocks. 1. 1967–1969. Avian Dis 1970; 14: 638–96.CrossRefGoogle ScholarPubMed
21.De Smedt, JM, Bolderdijk, RF. Dynamics of Salmonella isolation with modified semi-solid Rappaport-Vassiliadis medium. J Food Protect 1987; 50: 658–61.CrossRefGoogle ScholarPubMed
22.Van Leusden, FM, Van Schothorst, M, Beckers, HJ. The standard salmonella isolation method. In: Corry, JEL, Roberts, D, Skinner, FA, eds. Isolation and identification methods for food poisoning organisms. London: Society for applied bacteriology technical series no. 17, Academic Press, 1982: 35–49.Google Scholar
23.Fey, H, Burgi, E, Margadant, A, Boller, E. An economic and rapid diagnostic procedure for the detection of Salmonella/Shigella using the polyvalent Salmonella phage 0–1. Zentralbl Bacteriol Mikrobiol Hyg I Abt Orig A 1978; 240: 7–15.Google Scholar
24.Gudel, F, Fey, H. Improvement of the polyvalent Salmonella phage'ps 0–1 diagnostic value by addition of a phage specific for the O groups E1–E4. Zentralbl Bakteriol Mikrobiol Hyg I Abt Orig A 1981; 249: 220–4.Google Scholar
25.Crosa, JH, Falkow, S. Plasmids. In: Gerhardt, P, ed. Manual of methods for general bacteriology. Washington: American Society for Microbiology, 1981: 267–8.Google Scholar
26.Maniatis, TE, Fritsch, EF, Sambrook, J. Molecular cloning: a laboratory manual. Cold Spring Harbor: Cold Spring Harbor Laboratory, 1982: 97–106.Google Scholar
27.Sanderson, KE, Kadam, SK, MacLachlan, PR. Derepression of F factor function in Salmonella typhimurium. Can J Microbial 1983: 29: 1205–12.CrossRefGoogle ScholarPubMed
28.Macrina, FL, Kopecko, DJ, Jones, KR, Ayers, DJ, McCowen, SM. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1978; 1: 417–20.CrossRefGoogle ScholarPubMed
29. Anonymous. Salmonella serology. In: Difco Manual. 5th ed.Detroit: Difco Laboratories, 1984; 784–837.Google Scholar
30.Shipp, CR, Rowe, B. A mechanised microtechnique for Salmonella serotyping. J Clin Pathol 1980; 33: 595–7.CrossRefGoogle ScholarPubMed
31.Ward, LR, de Sa, JDH, Rowe, B. A phage-typing scheme for Salmonella enteritidis. Epidemiol Infect 1987; 99: 291–4.CrossRefGoogle ScholarPubMed
32. Anonymous. Methods for the isolation and identification of Salmonella from foods. Montreal: Polyscience Publications Inc. 1989. (Compendium of analytical methods; vol 2; HPB methods of microbiological analysis of foods; identification number MFHPB-20).Google Scholar
33. Anonymous. Official method for the determination of Salmonella. In: Laboratory Manual – Dry Milk Products. Agriculture Canada, Food Production and Inspection Branch, Ottawa, Ontario, 1988: pp DFV 9·1 and 9·2.Google Scholar
34.Snedecor, GW, Cochran, WG. Statistical methods, 7th ed.Ames IA: The Iowa State University Press, 1982: 124–128.Google Scholar
35.Milner, KC, Shaffer, MF. Bacteriological studies of experimental Salmonella infections in chicks. J Infect Dis 1952; 90: 81–96.Google Scholar
36.Sadler, WW, Brownell, JR, Fanelli, MJ. Influence of age and inoculum level on shed pattern of Salmonella typhimurium in chickens. Avian Dis 1969; 13: 793–803.CrossRefGoogle ScholarPubMed
37.Higgins, R, Malo, R, Rene-Roberge, E, Gauthier, R. Studies on the dissemination of Salmonella in nine broiler-chicken flocks. Avian Dis 1982; 26: 26–33.CrossRefGoogle ScholarPubMed
38.Barrow, PA, Tucker, JF, Simpson, JM. Inhibition of colonization of the chicken alimentary tract with Salmonella typhimurium gram-negative facultatively anaerobic bacteria. Epidemiol Infect 1987; 98: 311–22.CrossRefGoogle ScholarPubMed
39.Barnhart, H, Dreesen, DW, Bastien, RW, Pancorbo, OC. Prevalence of systemic Salmonella infection in layer hens at time of slaughter. In: Morello, JA, Domer, JE, eds. Abstracts of the 90th annual meeting of the American Society for Microbiology, Washington: American Society for Microbiology, 1990: 280.Google Scholar
40.Weisse, P, Libbey, E, Nims, L et al. , Salmonella heidelberg outbreak at a convention – New Mexico. Morb Mort Weekly Rep 1986; 35: 91.Google Scholar
41.Perales, I, Audicana, A. The role of hens' eggs in outbreaks of salmonellosis in north Spain. Int J Food Microbial 1989; 8: 175–80.CrossRefGoogle ScholarPubMed
42.Hinton, M, Pearson, GR, Threlfall, EJ, Rowe, B, Woodward, M, Wray, C. Experimental Salmonella enteritidis infection in chicks. Vet Rec 1989; 124: 223.CrossRefGoogle ScholarPubMed
43.Timoney, JF, Shivapradad, HL, Baker, RC, Rowe, B. Egg transmission after infection of hens with Salmonella enteritidis phage type 4. Vet Rec 1989; 125: 600–1.Google ScholarPubMed