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A New Method of obtaining Cultures from Single Bacterial Cells

Published online by Cambridge University Press:  15 May 2009

W. W. C. Topley ,
J. E. Barnard  and
G. S. Wilson
W. W. C. Topley
Affiliation:
From the Institute of Pathology, Charing Cross Hospital.
J. E. Barnard
Affiliation:
From the Institute of Pathology, Charing Cross Hospital.
G. S. Wilson
Affiliation:
From the Institute of Pathology, Charing Cross Hospital.
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While investigating variations in agglutinability, occurring among certain strains of paratyphoid bacilli, it became desirable to obtain cultures derived from single bacterial cells. The technique at first adopted was that devised by Mutch (1919), but the results obtained were not satisfactory. Certain modifications of this method were attempted, but were no more successful.

The main difficulty, and one which appears to us to have been far too little emphasised in all the methods hitherto described, has been encountered in obtaining satisfactory conditions for the microscopical observation of the bacillary suspensions employed. The difficulty of accurately observing hanging-drop preparations, and still more of controlling manipulation of them by microscopical observation, would seem to us to have been insufficiently realised; and the ease with which faulty interpretation of microscopical appearances may introduce fatal errors into work of this kind has not, perhaps, been fully taken into account.

Type
Research Article
Information
Journal of Hygiene , Volume 20 , Issue 3 , November 1921 , pp. 221 - 226
Copyright
Copyright © Cambridge University Press 1921

References

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