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Multiresistant serotype O 12 Pseudomonas aeruginosa: evidence for a common strain in Europe

Published online by Cambridge University Press:  15 May 2009

T.L. Pitt ,
D.M. Livermore ,
D. Pitcher ,
A.C. Vatopoulos  and
N.J. Legakis
T.L. Pitt
Affiliation:
Division of Hospital Infection
D.M. Livermore
Affiliation:
Department of Medical Microbiology, The London Hospital Medical College, Turner Street, London E1 2AD, UK
D. Pitcher
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
A.C. Vatopoulos
Affiliation:
Department of Microbiology, Hippocration General Hospital, 114 Vassilissis Sofias Avenue, Athens 11527, Greece
N.J. Legakis
Affiliation:
Department of Microbiology, School of Health Sciences, National University of Athens, P.O. Box 8540, 100 10 Athens, Greece
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A survey was made of serotype association and multiple antibiotic resistance in strains of Pseudomonas aeruginosa in Europe. Of 208 epidemiologically distinct strains from 16 laboratories in 10 countries, 48 were resistant to carbenicillin (MIC > 128 μg/ml) and gentamicin (MIC > 4 μ g/ml), and 12 of these strains were of serotvpe O 12. Representative O 12 strains from different countries were compared with two multiresistant O 12 strains isolated 5 years apart, from a British burns unit and the antibiotic sensitive serotype reference strain. All O 12 strains were similar by phage and pyocin typing but lysogenic phage profiles indicated that two strains (the later burns isolate and the serotype strain) were distinct from the others. Electrophoretic characterization of outer membrane proteins, lipopoly- saccharides and esterase enzymes corroborated the relationship of the strains and restriction fragment length polymorphism of DNA fragments hybridized with a cDNA probe copy of rRNA from P. aeruginosa provided further proof of their relatedness. We propose that the uniformity of characters of multiresistant O 12 P. aeruginosa in Europe is suggestive of a common origin for the strains.

Type
Research Article
Information
Epidemiology & Infection , Volume 103 , Issue 3 , December 1989 , pp. 565 - 576
Copyright
Copyright © Cambridge University Press 1989

References

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