Hostname: page-component-77c89778f8-rkxrd Total loading time: 0 Render date: 2024-07-17T11:09:56.048Z Has data issue: false hasContentIssue false
  • English
  • Français

Morphometrical parameters of gut microflora in human volunteers

Published online by Cambridge University Press:  15 May 2009

B. C. Meijer ,
G. J. Kootstra  and
M. H. F. Wilkinson
B. C. Meijer
Affiliation:
Laboratory for Medical Microbiology, State University of Groningen, Oostersingel 59, 9713 EZ Groningen, The Netherlands
G. J. Kootstra
Affiliation:
Laboratory for Medical Microbiology, State University of Groningen, Oostersingel 59, 9713 EZ Groningen, The Netherlands
M. H. F. Wilkinson
Affiliation:
Laboratory for Medical Microbiology, State University of Groningen, Oostersingel 59, 9713 EZ Groningen, The Netherlands
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The morphology of faecal microflora of nine healthy human volunteers was studied by digital image analysis of microscopic slides. Weekly specimens were collected during an 8-week period. Seven morphometrical parameters were derived: the means and medians of components 1, 2 and 3, and morphometrical entropy. Statistically significant differences among subjects were found for means of components 1 and 2, medians of components 1, 2 and 3, and entropy. The stability in normal circumstances provides an excellent basis for the detection of pathological change in gut flora balance.

Type
Research Article
Information
Epidemiology & Infection , Volume 107 , Issue 2 , October 1991 , pp. 383 - 391
Copyright
Copyright © Cambridge University Press 1991

References

REFERENCES

1.Van der Waaij, D. Colonization resistance of the digestive tract – mechanism and clinical consequences. Nahrung 1987; 31: 507–17.CrossRefGoogle ScholarPubMed
2.Gustafsson, BE. The physiological importance of the colonic microflora. Scand J Gastroenterol 1982; 77: 117–31.Google ScholarPubMed
3.Meijer-Severs, GJ, Van Santen, E, Meijer, BC. Short-chain fatty acid and organic acid concentrations in feces of healthy human volunteers and their correlation with anaerobe cultural counts during svstemic ceftriaxone administration. Scand J Gastroenterol 1990: 25: 698–704.CrossRefGoogle Scholar
4.Meijer-Severs, GJ, Van Santen, E. Short-chain fatty acid and organic acid concentrations in feces of 10 human volunteers and their correlation with anaerobe cultural counts over a 15-month period. Scand J Gastroenterol 1989; 24: 1276–80.CrossRefGoogle Scholar
5.Welling, GW, Stege, GJJ, Meijer-Severs, GJ. β-Aspartylpeptidase activity, a microflora associated characteristic (MAC); its presence in different strains of anaerobic bacteria. Microb Ecol Health Dis 1987; 1: 45–9.Google Scholar
6.Leijonmark, CE, Bergstrand, O, Carlstedt-Duke, B, et al. Total parenteral nutrition and the function of the intestinal microflora in Crohn's disease. Scand J Gastroenterol 1988; 23: 59–64.CrossRefGoogle Scholar
7.Moore, WEC, Holdeman, LV. Human faecal flora: the normal flora of 20 Japanese Hawaiians. Appl Environ Microbiol 1974; 27: 961–79.CrossRefGoogle ScholarPubMed
8.Apperloo-Renkema, HZ, van der Waaij, BD, van der Waaij, D. Determination of colonization resistance of the digestive tract by biotyping of Enterobacteriaceae. Epidemiol Infect 1990: 105: 355–61.CrossRefGoogle ScholarPubMed
9.Getliff, JM, Fry, JC. Using the Solitaire Plus image analyser for direct estimates of bacterial volume. Binary Comp Microbiol 1990; 2: 55–7.Google Scholar
10.Krambeck, C, Krambeck, H, Schröder, D, Newell, SY. Sizing bacterioplankton: a juxtaposition of bias due to shrinkage, halos, subjectivity in image interpretation and asymmetric distributions. Binary Comp Microbiol 1990; 2: 5–14.Google Scholar
11.Thomas, C, Packer, H. Morphological and structural measurements on filamentous microorganisms. Binary Comp Microbiol 1990; 2: 47–54.Google Scholar
12.Wynn-Williams, DD. The application of image analysis to natural terrestrial ecosystems. Binary Comp Microbiol 1990; 2: 15–20.Google Scholar
13.Gonzalez, RC, Wintz, P. Digital image processing. Reading, Massachusetts: Addison-Wesley, 1977.Google Scholar
14.Serra, J. Image analysis and mathematical morphology. London: Academic Press, 1982.Google Scholar
15.Meijer, BC, Kootstra, GJ, Wilkinson, MHF. A theoretical and practical investigation into the characterization of bacterial species by image analysis. Binary Comp Microbiol 1990; 2: 21–31.Google Scholar
16.Baquero, F, Fernandez-Jorge, A, Vicente, MF, Alós, JI, Reig, M. Diversity analysis of the human intestinal flora: a simple method based on bacterial morphotypes. Microb Ecol Health Dis 1988; 1: 101–8.Google Scholar
17.Kittler, J, Illingworth, J, Föglein, J. Threshold selection based on a simple image statistic. Comp Vision Graph Image Proc 1985; 30: 125–47.CrossRefGoogle Scholar
18.Mardia, KV, Kent, JT, Bibby, JM. Multivariate analysis. London: Academic Press, 1979.Google Scholar