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Molecular epidemiology of the plasmid-encoded TEM-1 β-lactamase in Scotland

Published online by Cambridge University Press:  15 May 2009

C. J. Thomson  and
S. G. B. Amyes
C. J. Thomson
Affiliation:
Scottish Antibiotic Reference Laboratory, Department of Medical Microbiology, The Medical School, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG. Scotland
S. G. B. Amyes
Affiliation:
Scottish Antibiotic Reference Laboratory, Department of Medical Microbiology, The Medical School, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG. Scotland
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A survey of the β-lactamases responsible for ampicillin resistance in urinary Escherichia coli isolated in central Scotland has been performed. The TEM-1 β-lactamase was found to be most prevalent occurring in 88.2% of ampicillin-resistant isolates tested. Forty-six percent of the TEM-1 producing strains were able to transfer this resistance to E. coli J62–2 at 37 ° C. Analysis of the resulting transconjugants revealed that the degree of resistance to amoxycillin and amoxycillin in combination with clavulanic acid was related to the specific activity of the TEM-1 β-lactamase. The variation in specific activity was shown to be related to plasmid type as determined by restriction analysis. No obvious relationship between β-lactamase specific activity and resistance to amoxycillin and amoxycillin plus clavulanic acid could be demonstrated in the original plasmid donor strains.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 1 , February 1993 , pp. 117 - 125
Copyright
Copyright © Cambridge University Press 1993

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