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Molecular epidemiology of plasmid patterns in Shigella flexneri types 1–6

Published online by Cambridge University Press:  15 May 2009

A. Gebre-Yohannes  and
B. S. Drasar
A. Gebre-Yohannes
Affiliation:
National Research Institute of Health, P.O. Box 1242, Addis Ababa, Ethiopia
B. S. Drasar
Affiliation:
Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
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A total of 123 drug-resistant and drug-sensitive Shigella flexneri types 1–6, and their Escherichia coli K12 transconjugants were used for plasmid profile analysis by agarose gel electrophoresis. Resistance factors (R-factors) were further characterized by incompatibility testing.

The overall distribution of small plasmids in S. flexneri showed that a cryptic plasmid of about 4·6 Kb was found in all serotypes, and a plasmid of about 4·2 Kb was found in serotypes 1–4. Shigella flexneri types 2, 4 and 6 showed a 6·5 Kb plasmid which correlated with SSu-resistance. All S. flexneri serotypes harboured large plasmids of about 217 Kb. Plasmid profile analysis of S. flexneri in Ethiopia showed a high degree of uniformity within individual serotypes. However, there was a limited variability which, at times, could be useful for epidemiological investigation. Shigella flexneri serotypes 1–6 harboured resistance plasmids with diverse molecular weights but mostly belonging to incompatibility groups N and X.

Type
Research Article
Information
Epidemiology & Infection , Volume 107 , Issue 2 , October 1991 , pp. 321 - 334
Copyright
Copyright © Cambridge University Press 1991

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