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Measurement of IgA responses following Norwalk virus infection and other human caliciviruses using a recombinant Norwalk virus protein EIA

Published online by Cambridge University Press:  15 May 2009

S. P. Parker  and
W. D. Cubitt
S. P. Parker
Affiliation:
Department of Virology, Institute of Child Health and Hospital for Sick Children, 30, Guilford Street, London WC1 1EH
W. D. Cubitt
Affiliation:
Department of Virology, Institute of Child Health and Hospital for Sick Children, 30, Guilford Street, London WC1 1EH
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Summary

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An enzyme immunoassay employing recombinant Norwalk virus capsid protein was evaluated for the measurement of IgA responses. Tests on 23 volunteers and patients known to have been infected with Norwalk virus (NV) showed that 19 developed significant IgA responses, 2 had unchanging levels of IgA and 2 failed to respond. There was no evidence of IgA responses to NV following infection with Hawaii or Snow Mountain-like viruses.

Tests on sera from patients involved in outbreaks associated with eating contaminated shellfish suggest that some patients may have been infected with more than one strain of calicivirus. The use of the rNV EIA for measuring IgA and IgG responses in patients involved in a major outbreak of food poisoning affecting hospital staff indicated that the causative agent was probably NV.

Type
Research Article
Information
Epidemiology & Infection , Volume 113 , Issue 1 , August 1994 , pp. 143 - 151
Copyright
Copyright © Cambridge University Press 1994

References

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