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Isolation of Legionella pneumophila from water supplies: comparison of methods based on the guinea-pig and culture media

Published online by Cambridge University Press:  19 October 2009

R. B. Fitzgeorge  and
P. J. Dennis
R. B. Fitzgeorge
Affiliation:
PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 OJG, UK
P. J. Dennis
Affiliation:
PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 OJG, UK
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Summary

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The detection of Legionella pneumophila in water via guinea-pig intraperitoneal injection has been compared with direct isolation of these organisms on semiselective BCYEα media. Both techniques were of similar sensitivity, detecting as few as 102L. pneumophila in 10 ml of water. However, at this concentration, detection using guinea-pig intraperitoneal injection was via the indirect parameters of antibody production and immunofluorescent microscopy. Isolation of L. pneumophila from guinea-pig tissue for further investigation required > 107 organisms injected and in this respect is relatively much less sensitive than direct isolation on semiselective BCYEα media. Since L. micdadei and L. longbeachae are inhibited by one of the selective supplements used, other supplements and techniques need to be developed in order to avoid possible inhibition of other Legionella species.

Type
Research Article
Information
Journal of Hygiene , Volume 91 , Issue 2 , October 1983 , pp. 179 - 187
Copyright
Copyright © Cambridge University Press 1983

References

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