Hostname: page-component-76fb5796d-25wd4 Total loading time: 0 Render date: 2024-04-26T13:17:48.651Z Has data issue: false hasContentIssue false
  • English
  • Français

Isolation of an agent of the spotted fever group rickettsia from tick eggs in Madrid, Spain

Published online by Cambridge University Press:  15 May 2009

C. Herrero ,
C. Pelaz  and
C. Martín-Bourgon
C. Herrero
Affiliation:
Servicio de Bacteriología, Centro National de Microbiología, Virología e Inmunología Sanitarias, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain
C. Pelaz*
Affiliation:
Servicio de Bacteriología, Centro National de Microbiología, Virología e Inmunología Sanitarias, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain
C. Martín-Bourgon
Affiliation:
Servicio de Bacteriología, Centro National de Microbiología, Virología e Inmunología Sanitarias, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain
*
*Author for reprints.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Ticks recovered from dogs in rural villages around Madrid (Spain) were processed to isolate rickettsiae. One sample containing mixtures of ticks and four containing eggs, in which rickettsiae had been detected by indirect immunofluorescence with a human serum highly reactive to Rickettsia conorii, were decontaminated, homogenized and inoculated onto Vero cells. Two egg samples yielded a cytopathic agent that reacted positively by immunofluorescence. One sample (14H) was successfully subcultured and identified as a member of the spotted fever group rickettsia. Tick eggs provide suitable material for isolation of rickettsia.

Type
Research Article
Information
Epidemiology & Infection , Volume 108 , Issue 3 , June 1992 , pp. 555 - 557
Copyright
Copyright © Cambridge University Press 1992

References

REFERENCES

1.Editorial. Fiebre botonosa en España 1983–1987. Bol Microbiol Sem 1988; 8: 1–3.Google Scholar
2.Otero, R, Fenoll, A, Casal, J. Resurgence of Mediterranean Spotted Fever. Lancet 1982; ii: 1107.CrossRefGoogle Scholar
3.Segura, F, Font, B. Resurgence of Mediterranean spotted fever in Spain. Lancet 1982; ii: 280.CrossRefGoogle Scholar
4.Espejo-Arenas, E, Raoult, D. First isolates of Rickettsia conorii in Spain using a centrifugation-shell vial assay. J Infect Dis 1989; 159: 1158–9.CrossRefGoogle ScholarPubMed
5.Herrero, C, Pelaz, C, Alvar, J et al. , Evidence of the presence of spotted fever group rickettsiae in dogs and dog ticks of the central provinces in Spain. Europ J Epidemiol 1992; In press.CrossRefGoogle ScholarPubMed
6.Hayes, SF, Burdgorfer, W. Reactivation of Rickettsia rickettsii in Dermacentor andersoni ticks: an ultrastructural analysis. Infect Immun 1982; 37: 779–85.CrossRefGoogle ScholarPubMed
7.Breitschwerdt, EB, Meuten, DJ, Walker, DH et al. , Canine Rocky Mountain spotted fever: a kennel epizootic. Am J Vet Res 1985; 46: 2124–8.Google ScholarPubMed
8.Anacker, RL, Mann, RE, Gonzales, C. Reactivity of monoclonal antibodies to Rickettsia rickettsii with Spotted Fever and Typhus group rickettsiae. J Clin Microbiol 1987; 25: 167–71.CrossRefGoogle ScholarPubMed
9.Philip, RN, Casper, E. Serotypes of spotted fever group rickettsiae isolated from Dermacentor andersoni (stiles) ticks in western Montana. Am J Trop Med Hyg 1981; 30: 230–8.CrossRefGoogle ScholarPubMed