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Gentamicin-resistant Salmonella typhimurium phage type 204c: molecular studies and strain diversity in a putative bovine outbreak

Published online by Cambridge University Press:  15 May 2009

D. J. Platt  and
I. Smith
D. J. Platt
Affiliation:
University Department of Bacteriology, Royal Infirmary, Glasgow G4 0SF, Scotland
I. Smith
Affiliation:
Veterinary Investigation Centre, West of Scotland Agricultural College, Auchincruive, Ayrshire
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As part of the investigation of a putative bovine outbreak, 13 isolates of Salmonella typhimurium phage type 204c were subjected to plasmid analysis.Plasmid profiles suggested that several distinct strains were involved and these observations were supported by minor variations in antibiotic resistance pattern. Restriction enzyme fingerprinting and conjugational segregation of the plasmids confirmed these findings. Although 12 of the 13 isolates were resistant to gentamicin, resistance was conferred by 4 distinct plasmids; 3 of these belonged to Inc I and were distantly related on the basis of restriction fingerprints and the fourth was a resistant derivative of the 60 MDa S. typhimurium serotype-specific plasmid. The molecular evidence refuted the hypothesis that geographical and temporal clustering of these gentamicin-resistant isolates could be explained on the basis of a single epidmiological episode.

Type
Research Article
Information
Epidemiology & Infection , Volume 107 , Issue 1 , August 1991 , pp. 213 - 223
Copyright
Copyright © Cambridge University Press 1991

References

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