Hostname: page-component-7479d7b7d-qlrfm Total loading time: 0 Render date: 2024-07-08T17:14:01.234Z Has data issue: false hasContentIssue false
  • English
  • Français

Genotypic and phenotypic analysis of Streptococcus uberis isolated from bovine mammary secretions

Published online by Cambridge University Press:  15 May 2009

B. M. Jayarao ,
S. P. Oliver ,
J. R. Tagg  and
K. R. Matthews
B. M. Jayarao
Affiliation:
Department of Animal Science, Institute of Agriculture, University of Tennessee, Knoxville, Tennessee, 37901-1071, USA
S. P. Oliver*
Affiliation:
Department of Animal Science, Institute of Agriculture, University of Tennessee, Knoxville, Tennessee, 37901-1071, USA
J. R. Tagg
Affiliation:
Department of Microbiology, University of Otago, Dunedin, New Zealand
K. R. Matthews
Affiliation:
Department of Animal Science, Institute of Agriculture, University of Tennessee, Knoxville, Tennessee, 37901-1071, USA
*
*Author for Correspondence
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Genotypic and phenotypic analysis of 42 strains of Streptococcus uberis isolated from mammary secretions of 17 cows collected at different periods of the lactation cycle and from episodes of clinical mastitis were performed. Seventeen restriction endonuclease fingerprint (REF) patterns and 12 bacteriocin-like inhibitory substance (BLIS) fingerprints were observed. REF identified and differentiated closely related strains of S. uberis isolated from mammary secretions collected from the same cow at different periods of the lactation cycle and from episodes of clinical mastitis. BLIS fingerprinting of S. uberis complemented REF results. REF and BLIS fingerprinting provided evidence concerning persistence of infection in the same quarter or different quarters of the mammary gland over different periods of the lactation cycle, and occurrence of infection with similar and dissimilar strains of S. uberis. Biochemical profiles could not identify closely related strains nor did they complement REF results. Antibiotic resistance patterns alone were of little value in differentiating closely related strains, but were identical with isolates having same REF pattern. None of the S. uberis strains was found to carry plasmids. REF and BLIS fingerprinting can be utilized effectively to differentiate closely related and unrelated strains of S. uberis isolated from bovine mammary secretions.

Type
Research Article
Information
Epidemiology & Infection , Volume 107 , Issue 3 , December 1991 , pp. 543 - 555
Copyright
Copyright © Cambridge University Press 1991

References

REFERENCES

1.Oliver, SP, Mitchell, BA. Prevalence of mastitis pathogens in herds participating in a mastitis control program. J Dairy Sci 1984; 67: 2436–40.CrossRefGoogle Scholar
2.Oliver, SP. Frequency of isolation of environmental mastitis causing pathogens and incidence of new intramammary infection during the nonlactating period. Am J Vet Res 1988: 49: 1789–93.Google ScholarPubMed
3.Oliver, SP, Sordillo, LM. Udder health in the periparturient period. J Dairy Sci 1988; 71: 2584–606.CrossRefGoogle ScholarPubMed
4.Smith, KL, Todhunter, DA, Schoenberger, PS. Environmental pathogens and intramammarv infection during the dry period. J Dairy Sci 1985: 68: 402–17.CrossRefGoogle ScholarPubMed
5.Bramlev, AJ, King, JS, Higgs, TM. The isolation of Streptococcus uberis from cows in two dairy herds. Br Vet J 1979; 135: 262–70.CrossRefGoogle Scholar
6.Hill, AW, Leigh, JA. DNA fingerprinting of Streptococcus uteris: a useful tool for epidemiology of bovine mastitis. Epidemiol Infect 1989; 103: 165–71.CrossRefGoogle Scholar
7.Hill, AW, Brady, CA. A note on the isolation and propagation of lytic phages from Streptococcus uberis and their potential for strain typing. J Appl Bact 1989: 67: 425–31.CrossRefGoogle ScholarPubMed
8.Tagg, JR, Bannister, LV. Fingerprinting of β hemolytic streptococci by their production of and sensitivity to bacteriocin like inhibitors. J Med Microbiol 1979: 12: 397–411.CrossRefGoogle Scholar
9.Caufield, PW, Walker, TM. Genetic diversity within Streptococcus mutatis evident from chromosomal DNA restriction fragment polymorphisms. J Clin Microbiol 1989; 27: 274–8.CrossRefGoogle Scholar
10.Geary, PP, Kalpan, EL, Livdhal, C, Skjold, S. DNA fingerprints of Streptococcus pyogenes are M type specific. J Infect Dis 1988: 158: 1317–23.Google Scholar
11.Denning, DW, Baker, CJ, Troup, N, Tompkins, LS. Restriction endonuclease analysis of human and bovine group B streptococci for epidemiologic study. J Clin Microbiol 1989; 27: 1352–6.CrossRefGoogle Scholar
12.Skjold, SA, Quie, PG, Fries, LA, Barnham, M, Cleary, PP. DNA fingerprinting of Streptococcus zooepidemicus (Lancefield Group C) as an aid to epidemiological study. J Infect Dis 1987: 155: 1145–50.CrossRefGoogle Scholar
13.Mogollon, JD, Pijoan, C, Murtaugh, MP, Kalpan, EL, Collins, JE, Cleary, PP. Characterization of prototype and clinically defined strains of Streptococcus suis by genomic fingerprinting. J Clin Mferobiol 1990; 28: 2462–6.CrossRefGoogle ScholarPubMed
14.Murray, BE, Singh, KV, Heath, JD, Sharma, BR, Weinstock, GM. Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites. J Clin Microbiol 1990; 28: 2059–63.CrossRefGoogle ScholarPubMed
15.Martin, NJ, Kalpan, EL, Gerber, MA et al. Comparison of epidemic and endemic group G streptococci by restriction enzyme analysis. J Clin Microbiol 1990: 28: 1881–6.CrossRefGoogle Scholar
16.Jayarao, BM, Oliver, SP, Matthews, KR, King, SH. Comparative evaluation of Vitek Gram-Positive Identification and API Rapid Strep Systems for identification of Streptococcus species of bovine origin. Vet Microbiol 1991; 26: 301–8.CrossRefGoogle ScholarPubMed
17.Bauer, AW, Kirby, WMM, Sherris, JC, Turck, M. Antibiotic susceptibility by standardized single disk method. Am J Clin Pathol 1966; 45: 493–6.CrossRefGoogle ScholarPubMed
18.LeBlanc, DJ, Lee, LN. Rapid screening procedure for detection of plasmids of streptococci. J Bacteriol 1979; 140: 1112–15.CrossRefGoogle Scholar
19.Anderson, DG, McKay, LL. Simple and rapid method for isolating plasmid DNA from lactic streptococci. Appl Environ Microbiol 1983; 46: 549–52.CrossRefGoogle ScholarPubMed
20.Meyers, JA, Sanchez, D, Elwell, LP, Falkow, S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol 1976: 127: 1529–37.CrossRefGoogle ScholarPubMed
21.Stableforth, AW. Streptococcal diseases of animals. In: Stableforth, AW, Galloway, IE, eds. Diseases due to bacteria, vol. 2. London: Butterworths Scientific Publications. 1959: 589–650.Google Scholar
22.Roguinskv, M. Reaction de Streptococcus uberis avee les serums G et P. Annales de I'institut Pasteur 1969; 117: 529–32.Google Scholar
23.Roguinskv, M. Comparaison de Streptococcus uberis et de Streptocoques voisins. Ouest Med 1972; 25: 983–5.Google Scholar
24.Garvie, EI, Bramlev, AJ. Streptococcus uberis: an approach to its classification. J Appl Bacterid 1979; 46: 295–304.CrossRefGoogle Scholar
25.Facklam, RR. Physiological differentiation of viridans streptococci. J Clin Microbiol 1977; 5: 184–201.CrossRefGoogle ScholarPubMed
26.Tagg, JR, Martin, DR. Bacteriocin ‘fingerprinting’ of group B Streptococcus strains of bovine and human origin. In: Proceedings of the University of Otago Medical School. 1980; 58: 22–3.Google Scholar
27.Schofield, CR, Tagg, JR. Bacteriocin-like activity of group B and C streptococci of human and of animal origin. J Hyg 1983; 90: 7–18.CrossRefGoogle Scholar
28.Tagg, JR, Vugler, LG. An inhibitor typing scheme for Streptococcus uberis. J Dairy Res 1986; 53: 451–6.CrossRefGoogle ScholarPubMed
29.Buddie, BM, Tagg, JR, Ralston, MJ. Use of inhibitor typing scheme to study the epidemiology of Streptococcus uberis mastitis. NZ Vet J 1988: 36: 115–19.Google Scholar
30.Tompkins, LS, Troup, NJ, Woods, T, Bibb, W, McKinney, RM. Molecular epidemiology of Legionella species by restriction endonuclease and alloenzyme analysis. J Clin Microbiol 1987: 25: 1875–80.CrossRefGoogle ScholarPubMed
31.Williams, AM, Collins, MD. Molecular taxonomic studies on Streptococcus uberis types I and II. Description of Streptococcus parauberis sp. nov. J Appl Bacteriol 1990; 68: 485–90.CrossRefGoogle ScholarPubMed