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Genetic relatedness of selected clinical Vibrio cholerae O139 isolates from the southern coastal area of China over a 20-year period

  • B. S. LI (a1) (a2), Y. XIAO (a3), D. C. WANG (a4) (a5), H. L. TAN (a1) (a2), B. X. KE (a1) (a2), D. M. HE (a1) (a2), C. W. KE (a1) (a2) and Y. H. ZHANG (a1) (a2)...

Summary

Vibrio cholerae O139 emerged as a causative agent of epidemic cholera in 1992 in India and Bangladesh, and was subsequently reported in China in 1993. The genetic relatedness and molecular characteristics of V. cholerae O139 in Guangdong Province, located in the southern coastal area of China, remains undetermined. In this study, we investigated 136 clinical V. cholerae O139 isolates from 1993 to 2013 in Guangdong. By conventional PCR, 123 (90·4%) isolates were positive for ctxB, ace and zot. Sequencing of the positive amplicons indicated 113 (91·7%) isolates possessed the El Tor allele of ctxB (genotype 3); seven carried the classical ctxB type (genotype 1) and three harboured a novel ctxB type (genotype 5). With respect to tcpA, 123 (90·4%) isolates were positive for the El Tor allele. In addition, pulsed-field gel electrophoresis (with NotI digestion) differentiated the isolates into clusters A and B. Cluster A contained seven of the non-toxigenic isolates from 1998 to 2000; another six non-toxigenic isolates (from 1998 and 2007) and all of the toxigenic isolates formed cluster B. Our results suggest that over a 20-year period, the predominant O139 clinical isolates have maintained a relatively tight clonal structure, although some genetic variance and shift has occurred. Our data highlight the persistence of toxigenic V. cholerae O139 in clinical settings in the southern coastal area of China.

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Corresponding author

*Author for correspondence: Dr Y. H. Zhang, 160 Qunxian Road, Dashi Street, Panyu District, Guangzhou, Guangdong 511430, China. (Email: zyh@cdcp.org.cn)

References

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1. Albert, MJ, et al. Large outbreak of clinical cholera due to Vibrio cholerae non-O1 in Bangladesh. Lancet 1993; 341: 704.
2. Ramamurthy, T, et al. Emergence of novel strain of Vibrio cholerae with epidemic potential in southern and eastern India. Lancet 1993; 341: 703704.
3. Comstock, LE, et al. Cloning and sequence of a region encoding a surface polysaccharide of Vibrio cholerae O139 and characterization of the insertion site in the chromosome of Vibrio cholerae O1. Molecular Microbiology 1996; 19: 815826.
4. Li, M, et al. Comparative genomic analyses of the vibrio pathogenicity island and cholera toxin prophage regions in nonepidemic serogroup strains of Vibrio cholerae . Applied and Environmental Microbiology 2003; 69: 17281738.
5. Olsvik, O, et al. Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains. Journal of Clinical Microbiology 1993; 31: 2225.
6. Nair, GB, et al. New variants of Vibrio cholerae O1 biotype El Tor with attributes of the classical biotype from hospitalized patients with acute diarrhea in Bangladesh. Journal of Clinical Microbiology 2002; 40: 32963299.
7. Bhuiyan, NA, et al. Changing genotypes of cholera toxin (CT) of Vibrio cholerae O139 in Bangladesh and description of three new CT genotypes. FEMS Immunology and Medical Microbiology 2009; 57: 136141.
8. Zhang, P, et al. Novel ctxB variants of Vibrio cholerae O1 isolates, China. Infection Genetics and Evolution 2013; 20: 4853.
9. Zhang, P, et al. The 7th pandemic Vibrio cholerae O1 El Tor isolate in China has undergone genetic shifts. Journal of Clinical Microbiology 2014; 52: 964967.
10. Liu, J, et al. Diarrhea outbreak by Vibrio cholerae O139 in Keping Xinjiang [in Chinese]. Disease Surveillance 1993; 8: 238239.
11. Zhang, P, et al. A molecular surveillance reveals prevalence of Vibrio cholerae O139 isolates in China, 1993–2012. Journal of Clinical Microbiology 2014; 52: 11461152.
12. World Health Organization. Manual for Laboratory Investigations of Acute Enteric Infections. Edited by the Diarrhoeal Disease Control Programme. Geneva: World Health Organization, 1987, pp. 1113.
13. Shi, L, et al. Detection of genes encoding cholera toxin (cholera toxin), zonula occludens toxin (ZOT), accessory cholera enterotoxin (ACE) and heat stable enterotoxin (ST) in Vibrio mimicus clinical strains. Microbiology and Immunology 1998; 42: 823828.
14. Rivera, IN, et al. Genotypes associated with virulence in environmental isolates of Vibrio cholerae . Applied and Environmental Microbiology 2001; 67: 24212429.
15. Singh, DV, et al. Molecular analysis of Vibrio cholerae O1, O139, non-O1, and non-O139 strains: clonal relationships between clinical and environmental isolates. Applied and Environmental Microbiology 2001; 67: 910921.
16. Chow, KH, et al. Detection of RTX toxin gene in Vibrio cholerae by PCR. Journal of Clinical Microbiology 2001; 39: 25942597.
17. Centers for Disease Control and Prevention. Rapid standardized laboratory protocol for molecular subtyping of Vibrio cholerae by pulsed field gel electrophoresis (PFGE), 2006.
18. Son, MS, et al. Characterization of Vibrio cholerae O1 El Tor biotype variant clinical isolates from Bangladesh and Haiti, including a molecular genetic analysis of virulence genes. Journal of Clinical Microbiology 2011; 49: 37393749.
19. Raychoudhuri, A, et al. Classical ctxB in Vibrio cholerae O1, Kolkata, India. Emerging Infectious Diseases 2009; 15: 131132.
20. Tran, HD, et al. Multi-drug resistant Vibrio cholerae O1 variant El Tor isolated in northern Vietnam between 2007 and 2010. Journal of Medical Microbiology 2012; 61: 431437.
21. Tang, X, et al. Outbreak of cholera associated with consumption of soft-shelled turtles, Sichuan province, China, 2009 [in Chinese]. Chinese Journal of Epidemiology 2010; 31: 10501052.
22. Li, H, et al. Analysis of the source of a cholera outbreak caused by O139 Vibrio cholerae [in Chinese]. Disease Surveillance 2008; 23: 218220.
23. Qiu, X, et al. Investigation on a foodborne cholera O139 outbreak in a rural banquet and the turtle markets [in Chinese]. Practical Preventive Medicine 2013; 20: 171174.

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Genetic relatedness of selected clinical Vibrio cholerae O139 isolates from the southern coastal area of China over a 20-year period

  • B. S. LI (a1) (a2), Y. XIAO (a3), D. C. WANG (a4) (a5), H. L. TAN (a1) (a2), B. X. KE (a1) (a2), D. M. HE (a1) (a2), C. W. KE (a1) (a2) and Y. H. ZHANG (a1) (a2)...

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