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Frequency of naturally occurring antibody to influenza virus antigenic variants selected in vitro with monoclonal antibody

Published online by Cambridge University Press:  25 March 2010

A. Natali
Affiliation:
Institute of Microbiology, University of Parma, Italy
J. S. Oxford
Affiliation:
National Institute for Biological Standards and Control, Hampstead, London NW3
G. C. Schild
Affiliation:
National Institute for Biological Standards and Control, Hampstead, London NW3
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Summary

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Antigenic variants of A/Texas/77 (H3N2) virus were selected in vitro using monoclonal antibody to virus haemagglutinin (HA). The antigenic variants and parental A/Texas/77 viruses were used to evaluate the frequency of anti-HA antibodies in the sera of children and adults using single-radial-haemolysis (SRH) tests. Twenty to 41 % of selected sera from adults, which contained antibody to the parental A/Texas/77 virus, failed to react with the different antigenic mutant viruses. A higher proportion of sera from children (37–58%) failed to react with the antigenic variants. Certain human sera and particularly those of children would appear to possess a more limited antibody repertoire to influenza HA, potentially allowing new antigenic variants to escape neutralization and spread in the community.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1981

References

Dowdle, W. R., Millar, J. D., Schonberger, L. B., Ennis, F. A., Lamontague, J. R. (1980). Influenza immunization policies and practices in Japan. Journal of Infectious Diseases 141, 258.CrossRefGoogle ScholarPubMed
Gerhard, W. & Webster, R. G. (1978). Antigenic drift in influenza A viruses I. Selection and characterisation of antigenic mutants of A/PR/8/34(HON1) virus with monoclonal antibodies. Journal of Experimental Medicine 148, 383.CrossRefGoogle Scholar
Haaheim, L. R. (1980). Haemagglutination inhibition antibodies in human sera to an antigenic mutant of influenza A/Texas/1/77 (H3N2) virus obtained in vitro. Acta Pathologica Scandinavica 88, 351.Google Scholar
Kilbourne, E. D. (1975). In The Influenza Viruses and Influenza (ed., Kilbourne, E. D. pp. 483538. New York: Academic Press.Google ScholarPubMed
Kilbourne, E. D. (1978). Genetic dimorphism in influenza viruses: characterisation of stably associated haemagglutinin mutants differing in antigenicity and biological properties. Proceedings of the National Academy of Science (USA) 75, 6258.CrossRefGoogle ScholarPubMed
Koprowski, H., Gerhard, W. & Croce, C. M. (1977). Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Proceedings of the National Academy of Science USA 74, 2985.CrossRefGoogle ScholarPubMed
Laver, W. G., Air, G. M., Webster, R. G., Gerhard, W., Ward, C. W. & Dopheide, T. A. A. (1980). The mechanism of antigenic drift in influenza virus: sequence changes in the haemagglutinin of variants selected with monoclonal hybridoma antibodies. Philosophical Transactions of the Royal Society B 288, 313.Google ScholarPubMed
Laver, W. G., Gerhard, W., Webster, R. G., Frankel, M. E. & Air, G. M. (1979). Antigenic drift in type A influenza virus: Peptide mapping and antigenic analysis of A/PR/8/34 (HON1) variants selected with monoclonal antibodies. Proceedings of the National Academy of Science USA 76, 1425.CrossRefGoogle ScholarPubMed
Oxford, J. S., Haaheim, L. R., Slepushkin, A., Werner, J., Kuwert, E. & Schild, G. C. (1981). Strain specificity of serum antibody to the haemagglutinin of influenza A (H3N2) viruses in children following immunization or natural infection. Journal of Hygiene 86, 17.CrossRefGoogle ScholarPubMed
Schild, G. C., Pereira, M. S., & Chakraverty, P. (1975). Single-radial-haemolysis: a new method for the assay of antibody to influenza haemagglutinin. Bulletin of the World Health Organization 52, 43.Google ScholarPubMed
Yewdell, J. W., Webster, R. G. & Gerhard, W. U. (1979). Antigenic variation in three distinct determinants of an influenza type A haemagglutinin molecule. Nature 279, 246.CrossRefGoogle ScholarPubMed