Evaluation of 16S, map1 and pCS20 probes for detection of Cowdria and Ehrlichia species

M. T. E. P. ALLSOPP; C. M. HATTINGH; S. W. VOGEL; B. A. ALLSOPP

doi:10.1017/S0950268899002101

Abstract

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A panel of 16S ribosomal RNA gene probes has been developed for the study of the epidemiology of heartwater; five of these detect different cowdria genotypes, one detects five distinct genotypes; one detects any Group III Ehrlichia species other than Cowdria and one detects any Group II Ehrlichia species. These probes have been used on PCR-amplified rickettsial 16S rRNA genes from over 200 Amblyomma hebraeum ticks. Control ticks were laboratory-reared and either uninfected or fed on sheep experimentally infected with different cowdria isolates, field ticks were collected from animals in heartwater-endemic areas. All tick-derived DNA samples were also examined by PCR amplification and probing for two other cowdria genes (map1 and pCS20) which have previously been used for heartwater epidemiology. This paper describes the first direct comparison of all currently available DNA probes for heartwater-associated organisms.

Footnotes

This work was presented in part at the 4th Biennial meeting of the Society for Tropical Veterinary Medicine, held in Montpellier, France, 5–9 May 1997.

Crossref Citations

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Burridge, M. J. Simmons, L. A. Simbi, B. H. Peter, T. F. and Mahan, S. M. 2000. Evidence ofCowdria ruminantiumInfection (Heartwater) inAmblyomma sparsumTicks Found on Tortoises Imported into Florida. Journal of Parasitology, Vol. 86, Issue. 5, p. 1135.

Peter, Trevor F. Barbet, Anthony F. Alleman, Arthur R. Simbi, Bigboy H. Burridge, Michael J. and Mahan, Suman M. 2000. Detection of the Agent of Heartwater, Cowdria ruminantium , in Amblyomma Ticks by PCR: Validation and Application of the Assay to Field Ticks . Journal of Clinical Microbiology, Vol. 38, Issue. 4, p. 1539.

Allsopp, M. T. E. P. and Allsopp, B. A. 2001. Novel Ehrlichia Genotype Detected in Dogs in South Africa . Journal of Clinical Microbiology, Vol. 39, Issue. 11, p. 4204.

Allsopp, M. T. E. P. Dorfling, C. M. Maillard, J. C. Bensaid, A. Haydon, D. T. van Heerden, H. and Allsopp, B. A. 2001. Ehrlichia ruminantium Major Antigenic Protein Gene ( map1 ) Variants Are Not Geographically Constrained and Show No Evidence of Having Evolved under Positive Selection Pressure . Journal of Clinical Microbiology, Vol. 39, Issue. 11, p. 4200.

Collins, Nicola E. Allsopp, Maria T.E.P. and Allsopp, Basil A. 2002. Molecular diagnosis of theileriosis and heartwater in bovines in Africa. Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 96, Issue. , p. S217.

VAN HEERDEN, H. COLLINS, N. E. ALLSOPP, M.T.E. P. and ALLSOPP, B. A. 2002. Major Outer Membrane Proteins of Ehrlichia ruminantium Encoded by a Multigene Family. Annals of the New York Academy of Sciences, Vol. 969, Issue. 1, p. 131.

STEYN, H. C. VAN HEERDEN, H. ALLSOPP, M.T. E.P. and ALLSOPP, B. A. 2003. Variability of pCS20 Gene Sequences among Different Ehrlichia ruminantium Isolates. Annals of the New York Academy of Sciences, Vol. 990, Issue. 1, p. 723.

ALLSOPP, M.T. E.P. VAN HEERDEN, H. STEYN, H. C. and ALLSOPP, B. A. 2003. Phylogenetic Relationships among Ehrlichia ruminantium Isolates. Annals of the New York Academy of Sciences, Vol. 990, Issue. 1, p. 685.

Vannini, C. Petroni, G. Verni, F. and Rosati, G. 2005. A Bacterium Belonging to the Rickettsiaceae Family Inhabits the Cytoplasm of the Marine Ciliate Diophrys appendiculata (Ciliophora, Hypotrichia). Microbial Ecology, Vol. 49, Issue. 3, p. 434.

Faburay, B. Geysen, D. Munstermann, S. Taoufik, A. Postigo, M. and Jongejan, F. 2007. Molecular detection of Ehrlichia ruminantium infection in Amblyomma variegatum ticks in The Gambia . Experimental and Applied Acarology, Vol. 42, Issue. 1, p. 61.

Allsopp, M.T.E.P. and Allsopp, B.A. 2007. Extensive genetic recombination occurs in the field between different genotypes of Ehrlichia ruminantium. Veterinary Microbiology, Vol. 124, Issue. 1-2, p. 58.

Allsopp, M.T.E.P. Van Strijp, M.F. Faber, E. Josemans, A.I. and Allsopp, B.A. 2007. Ehrlichia ruminantium variants which do not cause heartwater found in South Africa. Veterinary Microbiology, Vol. 120, Issue. 1-2, p. 158.

Faburay, Bonto Geysen, Dirk Ceesay, Ansumana Marcelino, Isabel Alves, Paula M. Taoufik, Amar Postigo, Milagros Bell-Sakyi, Lesley and Jongejan, Frans 2007. Immunisation of sheep against heartwater in The Gambia using inactivated and attenuated Ehrlichia ruminantium vaccines. Vaccine, Vol. 25, Issue. 46, p. 7939.

Faburay, Bonto Jongejan, Frans Taoufik, Amar Ceesay, Ansumana and Geysen, Dirk 2008. Genetic diversity of Ehrlichia ruminantium in Amblyomma variegatum ticks and small ruminants in The Gambia determined by restriction fragment profile analysis. Veterinary Microbiology, Vol. 126, Issue. 1-3, p. 189.

Allsopp, Basil A. and McBride, Jere W. 2009. Genome Mapping and Genomics in Animal-Associated Microbes. p. 117.

Raliniaina, Modestine Meyer, Damien F. Pinarello, Valérie Sheikboudou, Christian Emboulé, Loic Kandassamy, Yane Adakal, Hassane Stachurski, Frédéric Martinez, Dominique Lefrançois, Thierry and Vachiéry, Nathalie 2010. Mining the genetic diversity of Ehrlichia ruminantium using map genes family. Veterinary Parasitology, Vol. 167, Issue. 2-4, p. 187.

Allsopp, Basil A. 2010. Natural history of Ehrlichia ruminantium. Veterinary Parasitology, Vol. 167, Issue. 2-4, p. 123.

Pascucci, Ilaria Dondona, Andrea Capobianco Cammà, Cesare Marcacci, Maurilia Di Domenico, Marco Lelli, Rossella Scacchia, Massimo Jago, Mark Khaiseb, Siegfried Hager, Anna Laure Tjipura-Zaire, Georgina and Caporale, Vincenzo 2011. Survey of Ixodid Ticks and Two Tick-Borne Pathogens in African Buffaloes,Syncerus caffer, from the Caprivi Strip, Namibia. Journal of Zoo and Wildlife Medicine, Vol. 42, Issue. 4, p. 634.

Esemu, Seraphine N. Ndip, Roland N. and Ndip, Lucy M. 2014. Genetic diversity of <i>Ehrlichia ruminantium</i> strains in Cameroon. Onderstepoort J Vet Res, Vol. 81, Issue. 1,

Teshale, S. Geysen, D. Ameni, G. Asfaw, Y. and Berkvens, D. 2015. Improved molecular detection of Ehrlichia and Anaplasma species applied to Amblyomma ticks collected from cattle and sheep in Ethiopia. Ticks and Tick-borne Diseases, Vol. 6, Issue. 1, p. 1.

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Evaluation of 16S, map1 and pCS20 probes for detection of Cowdria and Ehrlichia species

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