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Egg-grown and tissue-culture-grown variants of influenza A (H3N2) virus with special attention to their use as antigens in seroepidemiology

Published online by Cambridge University Press:  19 October 2009

R. Pyhälä ,
L. Pyhälä ,
M. Valle  and
K. Aho
R. Pyhälä
Affiliation:
National Public Health Institute, Mannerheimintie 166, SF-00280 Helsinki, Finland
L. Pyhälä
Affiliation:
National Public Health Institute, Mannerheimintie 166, SF-00280 Helsinki, Finland
M. Valle
Affiliation:
National Public Health Institute, Mannerheimintie 166, SF-00280 Helsinki, Finland
K. Aho
Affiliation:
National Public Health Institute, Mannerheimintie 166, SF-00280 Helsinki, Finland
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A field strain of influenza A (H3N2) virus isolated in embryonated eggs during the 1984–5 influenza outbreak (A/Finland/13/85E) was compared in an antigenic analysis with virus from the same clinical specimen isolated in MDCK cell cultures (A/Finland/13/85M). The M-virus appeared to be more sensitive to haemagglutination-inhibiting antibodies against heterologous viruses than did the Evirus. The results of propagation and plaque purification experiments support the hypothesis that a single clinical specimen may consist of distinct antigenic variant subpopulations promoted selectively by the host during isolation procedures. Receptor-binding properties are discussed as a possible explanation for this selectivity.

A set of 471 paired sera consisting of pre-epidemic and post-epidemic specimens taken from the same subjects in 1984–5 was studied for haemagglutinationinhibiting antibodies to six influenza A (H3N2) virus strains, including the E-virus and the M-virus from A/Finland/13/85. Of the antigens used, the M-virus detected significant antibody increases more frequently than did the E-virus (10·0 v. 5·9%). The superiority of the M-virus may rest primarily in its ability to pick out anamnestic antibody responses. Irrespective of this cross-reactivity, preepidemic antibody to the M-virus was fairly well associated with protection. In the set of sera (230 specimens) collected in summer 1985 to represent different age groups, the antibody status against the M-virus was significantly better than the status against the E-virus. The results suggest that, at least in some instances, antibody to MDCK-grown virus is a more accurate indicator of the immune status of a community than antibodies to egg-grown virus variants.

Type
Research Article
Information
Epidemiology & Infection , Volume 99 , Issue 3 , December 1987 , pp. 745 - 753
Copyright
Copyright © Cambridge University Press 1987

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