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The effect of pre-enrichment on recovery of Streptococcus agalactiae, Staphylococcus aureus and mycoplasma from bovine milk

Published online by Cambridge University Press:  15 May 2009

M. C. Thurmond ,
J. W. Tyler ,
D. M. Luiz ,
C. A. Holmberg  and
J. P. Picanso
M. C. Thurmond
Affiliation:
Departments of Medicine, School of Veterinary Medicine/University of California (Davis), 18830 Road 112, Tulare, CA 93274, USA
J. W. Tyler
Affiliation:
Departments of Clinical Pathology, School of Veterinary Medicine/University of California (Davis), 18830 Road 112, Tulare, CA 93274, USA
D. M. Luiz
Affiliation:
and the Departments of Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine/University of California (Davis), 18830 Road 112, Tulare, CA 93274, USA
C. A. Holmberg
Affiliation:
Departments of Pathology, School of Veterinary Medicine/University of California (Davis), 18830 Road 112, Tulare, CA 93274, USA
J. P. Picanso
Affiliation:
and the Departments of Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine/University of California (Davis), 18830 Road 112, Tulare, CA 93274, USA
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The study was conducted to determine whether pre-enrichment would increase sensitivity of detecting Streptococcus (Str.) aqalactiae, Staphylococcus (S.) aureus, and mycoplasma in bovine milk. Two procedures were followed, one involving direct inoculation of milk on bovine blood agar, and the other involving preenrichment in broth followed by inoculation on agar. Logistic regression was used to predict the probability of isolation as a function of culture procedure and two additional covariates. the California Mastitis Test (CMT) score of the milk and the type of sample (indicating sample storage temperature and herd mastitis status). A total of 13778 milk samples was cultured for each of the three bacteria. By using results of both direct inoculation and pre-enrichment, the probability of isolation compared to use of direct inoculation only and adjusted for effects of other variables was increased 3·6-fold for Str. agalactiae, 1·6-fold for S. aureus and 1·7- fold for mycoplasma. The probability of isolation for all three bacteria increased as the CMT score increased. For Str. agalactiae, there was a statistical interaction predicting that enrichment improved the odds of isolation more from milk with high CMT scores than from milk with low scores. Results indicate that preenrichment can substantially increase the sensitivity of bacteriological screening of dairy cows for mastitis caused by Str. agalactiae, S. aureus, and mycoplasma.

Type
Research Article
Information
Epidemiology & Infection , Volume 103 , Issue 3 , December 1989 , pp. 465 - 474
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

1.Bushnell, RB.Herd health approach to mastitis control and milk quality. J Am Vet Med Assoc 1980; 176: 746–50.Google ScholarPubMed
2.Bushnell, RB. The importance of hygienic procedures in controlling mastitis. Vet Clin North Am (Large animal pract) 1984; 361–70.Google Scholar
3.Blowey, RW.Mastitis monitoring in general practice. Vet Rec 1984; 114: 259–61.CrossRefGoogle ScholarPubMed
4.Neave, FK. Dodd, FH, Kingwill, RG. Westgarth, DR.Control of mastitis in the dairy herd by hygiene and management. J Dairy Sci 1969; 52: 696–707.CrossRefGoogle ScholarPubMed
5.Schalm, OW, Carroll, EJ, Jain, NC.Bovine mastitis. Philadelphia: Lea and Febiger. 1971; 215.Google Scholar
6.Jasper, DE, Jain, NC, Brazil, LH.Clinical and laboratory observations on bovine mastitis due to Mycoplasma. J Am Vet Med Assoc 1966; 148: 1017–29.Google Scholar
7.Bennet, RH, Jasper, DE.Systemic and local immune responses associated with mammary infections due to Mycoplasmo bovis: resistance and susceptibility in previously infected cows. Am J Vet Res 1977; 38: 1731–8.Google Scholar
8.Anonymous. procedures for use in the diagnosis of bovine mastitis. National Mastitis Council Inc., Ames: Carter Press. 1981; 9.Google Scholar
9.Carmichael, LE, Guthrie, RS, Fincher, MG, Field, LE, Johnson, SD, Linquist, WE. Bovine mycoplasma mastitis. Proc US Livestock Sanit Assoc. 67th Annual Meeting. Richmond. Carter Composition. 1963; 220–35.Google Scholar
10.Jasper, DE. Bovine mycoplasmal mastitis. Adv Vet Sci Comp Med. Cornelius, CE, Simpson, CF eds. San Francisco: Academic Press, 1981; 121–59.Google Scholar
11.Thurmond, MC, Holmberg, CA, Luiz, DM.Evaluation of a digitonin disk assay to discriminate between acholeplasma and mycoplasma isolates from bovine milk. Cornell Vet 1989; 79:71–81.Google ScholarPubMed
12.Schalm, OW, Noorlander, DO.Experiments and observations leading to development of the California mastitis test. J Am Vet Med Assoc 1957; 130: 199–204.Google ScholarPubMed
13.Engelman, L. Stepwise logistic regression. In BMDP statistical software. Dixon, WJ ed. Berkeley: University of California Press. 1985; 330–44.Google Scholar
14.Blood, DC, Radostits, OM, Henderson, JA.Veterinary medicine. London: Bailliere Tindall. 1983; 464.Google Scholar
15.Martin, SW.Estimating disease prevalence and the interpretation of screening test results. Prev Vet Med 1984; 2: 463–72.Google Scholar
16.Pattison, IH.The progressive pathology of bacterial mastitis. Vet Rec 1958; 70: 114–7.Google Scholar
17.Bashandy, EY, Heider, LE.The effects of freezing milk samples on the culture results. Zentrabl Veterinarmed [B] 1979; 26: 1–6.Google Scholar
18.Neave, FK, Phillips, M, Mattick, ATR.Clinical mastitis in six herds freed from Streptococcus agalactiae. J Dairy Res 1952; 19: 14–30.Google Scholar
19.Raccach, M, Rottem, S, Razin, S.Survival of frozen mycoplasma. Appl Microbiol 1975; 30: 167–71.Google Scholar
20.Jasper, DE, Dellinger, JD, Rollins, MH, Hakanson, HD.Prevalence of mycoplasmal bovine mastitis in California. Am J Vet Res 1979; 40: 1043–7.Google ScholarPubMed