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Drug resistance and plasmid profile of shigellae in Taiwan

Published online by Cambridge University Press:  15 May 2009

S.-R. Lin  and
S.-F. Chang
S.-R. Lin
Affiliation:
Department of Clinical Laboratory and Microbiology, No. 100 Shih-Chuan 1st Rd, Kaohsiung Medical College, Kaohsiung City, 80708, Taiwan, Republic of, China
S.-F. Chang
Affiliation:
Department of Clinical Laboratory and Microbiology, No. 100 Shih-Chuan 1st Rd, Kaohsiung Medical College, Kaohsiung City, 80708, Taiwan, Republic of, China
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Summary

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One hundred and twenty-eight shigella strains isolated from newborn and infant human faecal specimens at Kaohsiung Medical College Hospital in Taiwan were serogrouped, serotyped and examined for drug-resistance patterns and for the presence of plasmids. Forty-seven pre cent of the isolates were found to belong to the Shigella sonnei serogroup, 41%to the S.flexneri group,9%to the S.boydii group and 3%to the S.dysenteriae group.The serotype with the greatest number of strains was S.sonnei I. (29 %) followed by S. flexneri 1 (27%). Each strain was tested for resistance to 11 antimicrobial agents. Eighty-eight per cent of the strains were resistant to tetracycline, 87% to chloramphenicol, 84% to streptomycin, 52% to ampicillin, 25% to nalidixic acid, 29% to kanamycin, 11 % to cephalothin, 11% to neomycin, 10% to cotrimoxazole, 1% to amikacin and none to gentamicin. The most prevalent resistance pattern was ApCmSmTc (28%). Clinical isolates demonstrating multiple resistance were found to harbour a large transmissible plasmid of 45–75 MDa while isolates without multiple resistance did not. Two large virulence plasmids of 123 and 110 MDa were found in 12 strains of S. flexneri and 4 strains of S. sonnei phase I. Small plasmids of 4·5, 4·2, 3·5, 2·8, 2·5. 2·0 and 1·5 MDa were also present in all strains. These small plasmids were species specific and can be used as marker plasmids to identify species.

Type
Research Article
Information
Epidemiology & Infection , Volume 108 , Issue 1 , February 1992 , pp. 87 - 97
Copyright
Copyright © Cambridge University Press 1992

References

REFERENCES

1.Arora, DR, Midha, NK, Ichhpujani, RL, Chugh, TD. Drug resistant shigellosis in north India. Indian J Med Res 1982; 76: 74–9.Google ScholarPubMed
2.Olukoya, DK, Oni, O. Plasmid profile analysis and antimicrobial susceptibility patterns of shigella isolates from Nigeria. Epidemiol Infect 1990; 105: 59–64.CrossRefGoogle ScholarPubMed
3.Albert, MJ, Singh, KV, Murray, BE, Ehrlich, J. Molecular epidemiology of shigella infection in central Australia. Epidemiol Infect 1990; 105: 51–7.CrossRefGoogle ScholarPubMed
4.Wang, KY, Chang, SF, Peng, CF, Chen, JC. Studies on shigella isolated in Southern Taiwan. Chinese J Microbiol Immunol 1976; 9: 13–8.Google ScholarPubMed
5.Gedebou, M, Tassew, A. Shigella species from Addis Ababa: frequency of isolation and in vitro drug sensitivity. J Hyd 1982; 88: 47–55.Google Scholar
6.Suzuki, S, Nakazawa, S, Ushioda, T. Yearly changes of drug resistance of shigella strains isolated in Kyoto for five years from 1951. Chemotherapy 1986; 4: 336–40.Google Scholar
7.Chugh, TD, Suheir, A, Mahboob, AG, Neil, L, Bl-Bishbishi, E. Plasmid–mediated drug resistance of shigellae in Kuwait. Antonie van Leeuwenhoek 1985; 51: 241–7.CrossRefGoogle ScholarPubMed
8.Bauer, AW, Kirby, WM, Sherris, JC, Turck, M. Antibiotic susceptibility testing by a standard single disk method. Am J Clin Pathol 1966; 45:493–96.CrossRefGoogle ScholarPubMed
9.Steers, E, Foltz, EL, Graves, BS. Inocula replicating device apparatus for routing testing of bacteria; susceptibility to antibiotics. Antibiot Chemother 1973; 9: 307–11.Google Scholar
10.Lin, SR, Chang, SF, Chen, SS, Peng, CF. Molecular characterization of virulence plasmid in shigella species. Kaoshiung J Med Sci 1988; 4: 633–42.Google ScholarPubMed
11.Agarwal, SK, Goel, M, Das, R, Kumar, A. Transmissible antibiotic resistance among shigella species. Indian J Med Res 1984; 8; 402–8.Google Scholar
12.Macaden, R, Gokul, BN, Pereira, P, Bhat, P. Bacillary dysentary due to multidrug resistant S. dysenteriae type I. Indian J Med Res 1980; 71: 178–85.Google Scholar
13.Blaser, MJ, Pollard, RA, Feldman, RA. Shigella infections in the United States, 1974–1980. J Infect Dis 1983; 147: 771–5.Google ScholarPubMed
14.Jesudason, MV, Lalitha, MK, Koshi, G. Changes in incidence of shigella subgroups and their antibiotic susceptibility pattern in Vellore, South India. J Trop Med Hyg 1985; 88: 355–8.Google ScholarPubMed
15.Nigar, SS, Khaleda, H, David, AS. Shigellosis in Bangladesh: a reply. J Infect Dis 1978; 156: 535–9.Google Scholar
16.Donald, PR, Pretorius, ML, Burger, PJ. Shigellosis in the South-western cape of Good Hope 1968–85. Epidemiol Infect 1987; 98: 165–70.CrossRefGoogle ScholarPubMed
17.Yohannes, AG, Draser, BS. Plasmid mediated drug resistance in Shigella flexneri serotypes 1–6 during 1974–85. Indian J Med Res 1988; 88: 480–7.Google Scholar
18.Mel, D, Gangorosa, EJ, Radovanouic, MD, Arise, BL, Litinrinjanko, S. Studies of vaccination against bacillary dysentery 6. Protection of children by shigella strains. Bull WHO 1971; 45: 457–64.Google ScholarPubMed
19.Mutanda, LN, Kaviti, JN, Wamola, IA. Patterns of shigella species and serotypes in East Africa. East Afr Med J 1979; 56: 381–7.Google ScholarPubMed
20.Mutanda, Ln, Kibriya, JN, Wamola, LA. Patterns of shigella species and serotupes in East Africa. East AFr Med J 1979; 56: 381–7.Google ScholarPubMed
21.Kopecko, DJ, Washington, O, Formal, SB. Genetic and physical evidence for plasmid control of Shigella sonnei form I cell surface antigen. Infect Immun 1980; 29: 207–14.CrossRefGoogle ScholarPubMed
22.Sansonetti, PJ, Kopecko, J, Formal, SB. Shigella sonnei plasmids evidence that a large plasmid is necessary for virulence. Infect Immun 1981; 34: 75–83.CrossRefGoogle ScholarPubMed
23.Haider, K, Huq, MI, Samadi, AR, Ahmad, K. Plasmid characterization of Shigella Spp. isolated from children with shigellosis and asymptomatic excretors. J AntimicrobChemother 1985; 16: 691–8.Google ScholarPubMed
24.Sakaguchi, S, Sakaguchi, T, Arai, T. A survey of plasmid in the clinical isolated shigella strains and their invasive ability and antibiotic resistance. Kitasate Arch of Exp Med 1986; 59: 1–7.Google Scholar
25.Mata, LJ, Gangarasa, EJ, Caceres, A, Perea, DR, Mejicanos, LM. Epidemic Shiga bacillus dysentery in Central America. 1. Etilogic investigation, Guatemala in 1969. J Infect Dis 1970; 122: 170–80.CrossRefGoogle Scholar
26.Paniker, CKJ, Vimla, KN, Bhat, P, Stephen, S. Drug resistant shigellosis in South India. Indian J Med Res 1978; 68: 413–7.Google ScholarPubMed