Hostname: page-component-8448b6f56d-c47g7 Total loading time: 0 Render date: 2024-04-24T08:21:45.758Z Has data issue: false hasContentIssue false
  • English
  • Français

DNA fingerprinting of Streptococcus uberis: a useful tool for epidemiology of bovine mastitis

Published online by Cambridge University Press:  15 May 2009

A. W. Hill  and
J. A. Leigh
A. W. Hill
Affiliation:
AFRC Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berks RG16 ONN, UK
J. A. Leigh
Affiliation:
AFRC Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berks RG16 ONN, UK
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A simple and reproducible typing system based on restriction fragment size of chromosomal DNA was developed to compare isolates of Streptococcus uberis obtained from the bovine mammary gland. The endonuclease giving the most useful restriction patterns was Hind III, although seven other endonucleases (Bgl 1, Eco R1, Not 1, Pst 1, Sfi 1, Sma 1, Xba 1) were also tested in the system. An image analyser was used to obtain a densitometric scan and a graphic display of the restriction patterns. Such a system will allow large scale data storage for future computer-aided comparison.

Type
Special Article
Information
Epidemiology & Infection , Volume 103 , Issue 1 , August 1989 , pp. 165 - 171
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

1.Bramley, AJ, Dodd, FH. Reviews of the progress of dairy science: Mastitis control-progress and prospects. J Dairy Res 1984; 51: 481–512.CrossRefGoogle ScholarPubMed
2.Marshall, RB, Wilton, BE, Robinson, AJ. Identification of leptospira serovars by restriction endonuclease analysis. J Med Microbiol 1981: 14: 163–6.CrossRefGoogle ScholarPubMed
3.Kaper, JB, Bradfod, HB, Roberts, NC, Falkow, S.Molecular epidemiology of Vibrio cholerae in the U.S. Gulf Coast. J Clin Microbiol 1982; 16: 129–34.CrossRefGoogle ScholarPubMed
4.Kakoyiannis, CK, Winter, PJ, Marshall, RB. Identification of isolates of Campylobacter coli from animals and humans by bacterial restriction endonuclease DNA analysis (BRENDA). Appl Environ Microbiol 1984: 48: 545–9.CrossRefGoogle Scholar
5.Marshall, RB, Winter, PJ, Cooper, BS, Robinson, AJ. Subspecies differentiation of Morexella bovis by restriction endonuclease analysis (BRENDA). N Z Vet J 1985; 33: 67–70.CrossRefGoogle Scholar
6.Skjold, SA, Cleary, PP. DNA fingerprints of Group A, serotype M49 streptococci. In: Ferretti, JJ, Curtiss, R, eds. Streptococcal genetics. Washington, DC: American Society for Microbiology, 1987: 127–9.Google Scholar
7.Bramley, AJ. Streptococcus uberis mastitis. Epidemiology and pathogenesis. Anim Health Nutr 1987: 42: 12–6.Google Scholar
8.Kruze, J. The aetiology of bovine mastitis caused by Streptococcus uberis [Ph.D. Thesis]. Reading, UK: University of Reading, 1983. 256 pp.Google Scholar
9.Garvie, EI, Bramley, AJ. Streptococcus uberis: an approach to its classification. J Appl Bacteriol 1979: 46: 295–304.CrossRefGoogle Scholar
10.Miller, SA, Dykes, DD, Polesky, HFA simple salting out procedure for extracting DNA from human nucleate cells. Nucleic Acids Res 1988; 16: 1215.CrossRefGoogle Scholar
11.Tagg, JR, Vugler, LG. An inhibitor typing system for Streptococcus uberis. J Dairy Res 1986; 53: 451–6.CrossRefGoogle Scholar
12.Bautsch, W, Grothues, D, Tummler, B.Genome fingerprinting of Pseudomonas aeruginosa by two-dimensional field inversion gel electrophoresis. FEMS Microbiol Lett 1988; 52: 255–8.CrossRefGoogle Scholar
13.Burnie, JP, Lee, W.A comparison of DNA and immunoblot fingerprinting of the SII biotype of coagulase negative staphylococci. Epidemiol Infect 1988; 101: 203–12.CrossRefGoogle ScholarPubMed
14.Kakoyiannis, CK, Winter, PJ, Marshall, RB.The relationship between intestinal Campylobacter species isolated from animals and humans as determined by BRENDA. Epidemiol Infect 1988; 100: 379–87.CrossRefGoogle ScholarPubMed