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Distribution and characteristics of verocytotoxigenic Escherichia coli isolated from Ontario dairy cattle

Published online by Cambridge University Press:  15 May 2009

J. B. Wilson ,
S. A. McEwen ,
R. C. Clarke ,
K. E. Leslie ,
R. A. Wilson ,
D. Waltner-Toews  and
C. L. Gyles
J. B. Wilson
Affiliation:
Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
S. A. McEwen
Affiliation:
Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
R. C. Clarke
Affiliation:
Agriculture Canada, Health of Animals Laboratory, 110 Stone Road West, Guelph, Ontario, Canada, N1G 3W4
K. E. Leslie
Affiliation:
Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
R. A. Wilson
Affiliation:
Department of Veterinary Science, Pennsylvania State University, University Park, Pennsylvania 16802
D. Waltner-Toews
Affiliation:
Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
C. L. Gyles
Affiliation:
Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
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Faecal swabs obtained from a random sample of 1131 cows and 659 calves on 100 southern Ontario dairy farms were examined for verocytotoxigenic Escherichia coli (VTEC) using a Vero cell assay. Five isolates from each positive culture were tested similarly. Positive colonies were examined with DNA probes for Shiga-like toxin I (SLT-I) and SLT-II sequences. Probe-negative colonies were tested for neutralization of verocytotoxicity using anti-SLT-I and anti-SLT-IIv antisera. Colonies showing no neutralization response were examined in a polymerase chain reaction procedure. Colonies positive by any test were confirmed to be E. coli biochemically, serotyped, biotyped and tested for antimicrobial resistance. Faecal culture supernatants which were positive in the Vero cell assay, but culture negative, were examined using the neutralization assay. Animals were classified positive by faecal culture supernatant or by positive VTEC isolate. The prevalence rates of VTEC infection in cows and calves were estimated to be 9·5 and 24·7%, respectively. The proportion of animals infected on each farm ranged from 0 to 60% for cows and 0 to 100% for calves. Of 206 VTEC isolates identified, few were of serotypes which have been isolated from humans and none were E. coli O 157. H7.

Type
Research Article
Information
Epidemiology & Infection , Volume 108 , Issue 3 , June 1992 , pp. 423 - 439
Copyright
Copyright © Cambridge University Press 1992

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