Hostname: page-component-848d4c4894-hfldf Total loading time: 0 Render date: 2024-05-10T06:38:03.350Z Has data issue: false hasContentIssue false

Discrimination between endemic and feedborne Salmonella Infantis infection in cattle by molecular typing

Published online by Cambridge University Press:  01 June 1999

N. LINDQVIST
Affiliation:
National Veterinary and Food Research Institute, Kuopio Regional Laboratory, P.O. Box 92, FIN-70701 Kuopio, Finland
S. HEINIKAINEN
Affiliation:
National Veterinary and Food Research Institute, Kuopio Regional Laboratory, P.O. Box 92, FIN-70701 Kuopio, Finland
A.-M. TOIVONEN
Affiliation:
National Veterinary and Food Research Institute, Kuopio Regional Laboratory, P.O. Box 92, FIN-70701 Kuopio, Finland
S. PELKONEN
Affiliation:
National Veterinary and Food Research Institute, Kuopio Regional Laboratory, P.O. Box 92, FIN-70701 Kuopio, Finland
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Salmonella enterica serovar Infantis is endemic in Finnish cattle. Feed contaminated with S. Infantis was distributed to cattle farms in May 1995. Following increased sampling, S. Infantis was detected on 242 farms in 1995. Molecular typing was used to differentiate the farms that were infected by the feed-related Infantis from those infected by other endemic strains. Twenty-three isolates from feed in 1995 and 413 from cattle (72 from 1992–4, 324 from 1995, 17 from 1996–7) were analysed. The feed-related Infantis was clonally related to the endemic infection by the ribotype, IS200-type and XbaI-profile. The feed isolates had a distinctive plasmid that appeared in pulsed-field gel electrophoresis as a 60 kb band when cleaved with XbaI or linearized by S1-nuclease. This plasmid appeared in cattle only since the outbreak and seemed stable on the follow-up farms. In addition to contact farms, the feedborne strain was found on 19% of the farms infected with S. Infantis in 1995 but not having bought suspected feedstuffs, possibly as secondary infections.

Type
Research Article
Copyright
© 1999 Cambridge University Press