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Detection of endotoxins with the Limulus test in burned and unburned mice infected with different species of gramnegative bacteria

Published online by Cambridge University Press:  15 May 2009

R. J. Jones ,
E. A. Roe  and
R. E. Dyster
R. J. Jones
Affiliation:
MRC Industrial Injuries and Burns Unit, Accident Hospital, Bath Row, Birmingham, B15 1NA
E. A. Roe
Affiliation:
MRC Industrial Injuries and Burns Unit, Accident Hospital, Bath Row, Birmingham, B15 1NA
R. E. Dyster
Affiliation:
MRC Industrial Injuries and Burns Unit, Accident Hospital, Bath Row, Birmingham, B15 1NA
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Summary

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The Limulus test detected endotoxins in the plasma of burned and unburned mice infected with different species of gram-negative bacteria. Individual strains of different species of gram-negative bacteria produced different amounts of endotoxin in the plasma of infected mice. Plasma from mice given lethal infections showed very high concentrations of endotoxin. Low concentrations of endotoxin in the plasma were tolerated by mice but high concentrations were invariably fatal. A polyvalent pseudomonas vaccine reduced endotoxin in the plasma of mice given lethal infections of Pseudomonas aeruginosa.

Type
Research Article
Information
Journal of Hygiene , Volume 75 , Issue 1 , August 1975 , pp. 99 - 112
Copyright
Copyright © Cambridge University Press 1975

References

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