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Concentration and partial purification of poliomyelitis viruses

Published online by Cambridge University Press:  15 May 2009

K. H. Fantes
K. H. Fantes
Affiliation:
Biochemistry Section, Virus Unit, Glaxo Laboratories Ltd, ‘Sefton Park’, Stoke Poges, Bucks
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1. Conditions are described under which the three types of live or formalin-inactivated poliomyelitis viruses can be concentrated 10–100 times in good yield, with elimination of approximately 90% of the extraneous protein normally present in poliovaccines.

2. It was shown that 0·1–0·2 mg./ml. AlPO4 almost quantitatively adsorbed the antigens, provided the adsorptions were carried out at a pH round about 5. Re-elution of the antigens occurred readily at pH 7–pH 8.

3. No noticeable conversion of the ‘D-antigen’ to the ‘C-antigen’ occurred during the concentration.

Type
Research Article
Information
Journal of Hygiene , Volume 60 , Issue 1 , March 1962 , pp. 123 - 133
Copyright
Copyright © Cambridge University Press 1962

References

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