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The chemical reactions of the haemagglutinins and neuraminidases of different strains of influenza viruses: III. Effects of proteolytic enzymes

Published online by Cambridge University Press:  15 May 2009

L. Hoyle  and
June D. Almeida
L. Hoyle
Affiliation:
Public Health Laboratory, General Hospital, Northampton
June D. Almeida
Affiliation:
Virological Department, Royal Post-Graduate Medical School, Hammersmith Hospital, London
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Summary

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The action of trypsin and pronase on the haemagglutinins and neuraminidases of eight strains of influenza virus has been examined.

The haemagglutinins of all the strains were highly susceptible to digestion by pronase but there were great variations in resistance to trypsin.

The neuraminidases of the eight strains were of three types. The neuraminidases of the A1 strains and the DSP strain of virus A were highly susceptible to destruction by both enzymes. The neuraminidases of the PR 8 and Swine strains showed partial resistance especially to trypsin, while the A 2 strains and the lee strains of virus B possessed neuraminidases that were completely resistant to both trypsin and pronase.

Proteolytic enzymes released free neuraminidases from the A 2 and lee viruses the morphology of which was different from that of neuraminidases released by detergent treatment.

Type
Research Article
Information
Journal of Hygiene , Volume 69 , Issue 3 , September 1971 , pp. 461 - 468
Copyright
Copyright © Cambridge University Press 1971

References

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