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Characterization of multi-drug resistant Mycobacterium tuberculosis from immigrants residing in the USA using Ion Torrent full-gene sequencing

  • L. T. DAUM (a1), G. W. FISCHER (a1), J. SROMEK (a2), M. KHUBBAR (a2), P. HUNTER (a2) (a3), M. S. GRADUS (a2) and S. BHATTACHARYYA (a2)...

Summary

Drug-resistant Mycobacterium tuberculosis bacterium (MTB) is spreading worldwide. Three drug-resistant isolates were detected in Burmese, Hmong, and Indian immigrants currently residing in Milwaukee, Wisconsin, USA. Ion Torrent full-gene sequencing and complete genetic analysis was performed within 5 days and compared to results from traditional drug sensitivity testing (DST). Genetic characterization of seven, full-length resistance-associated genes revealed two MDR and one highly resistant strain with important drug-resistant mutations that were confirmed by traditional DST. The rapid turnaround from sample-to-sequence underscores the public health value of Ion Torrent full-gene sequencing of MDR/XDR genes from epidemiologically significant clinical isolates.

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Copyright

The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution-NonCommercial-ShareAlike licence . The written permission of Cambridge University Press must be obtained for commercial re-use.

Corresponding author

* Author for correspondence: Dr L. T. Daum, 1747 Citadel Plaza Suite 206, San Antonio, TX, 78209, USA. (Email: ltd@lhnvd.com)

References

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Keywords

Characterization of multi-drug resistant Mycobacterium tuberculosis from immigrants residing in the USA using Ion Torrent full-gene sequencing

  • L. T. DAUM (a1), G. W. FISCHER (a1), J. SROMEK (a2), M. KHUBBAR (a2), P. HUNTER (a2) (a3), M. S. GRADUS (a2) and S. BHATTACHARYYA (a2)...

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