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Characterization of human faecal flora by means of an improved fluoro-morphometrical method

Published online by Cambridge University Press:  15 May 2009

G.J. Jansen ,
M. H. F. Wilkinson ,
B. Deddens  and
D. Van Der Waaij
G.J. Jansen
Affiliation:
Laboratory of Medical Microbiology, University Hospital of Groningen, Oostersingel 59, 9713 EZ, Groningen, The Netherlands
M. H. F. Wilkinson
Affiliation:
Laboratory of Medical Microbiology, University Hospital of Groningen, Oostersingel 59, 9713 EZ, Groningen, The Netherlands
B. Deddens
Affiliation:
Laboratory of Medical Microbiology, University Hospital of Groningen, Oostersingel 59, 9713 EZ, Groningen, The Netherlands
D. Van Der Waaij
Affiliation:
Laboratory of Medical Microbiology, University Hospital of Groningen, Oostersingel 59, 9713 EZ, Groningen, The Netherlands
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Summary

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The use of polyclonal antibodies in differentiating between samples of faecal microflora derived from ten healthy volunteers was assessed. The distribution of FITC-labelled antibodies (of the IgA, IgG and IgM isotype) over 144 morphologically distinct subsets of faecal bacteria (‘morphotypes’) was quantified by means of digital morphometry and quantitative immunofluorescence. Furthermore, a new dataprocessing algorithm was developed which makes objective quantitation of the antibody distributions over the faecal morphotypes possible. The results of this study imply that the antibody binding capacity of faecal morphotypes is a unique characteristic of faecal microflora.

Type
Research Article
Information
Epidemiology & Infection , Volume 111 , Issue 2 , October 1993 , pp. 265 - 272
Copyright
Copyright © Cambridge University Press 1993

References

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