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Bismuth sulphite media for the isolation of V. cholerae

Published online by Cambridge University Press:  15 May 2009

W. James Wilson  and
L. V. Reilly
W. James Wilson
Affiliation:
From Public Health Laboratories, Queen's University, Belfast
L. V. Reilly
Affiliation:
From Public Health Laboratories, Queen's University, Belfast
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1. A fluid enrichment medium containing sulphite and bismuth based on Read's modification of one of Wilson & Blair's media has been devised and the claims of Read (1939) and Seal (1939) as to the value of such a medium has been confirmed.

2. A saccharose mannitol sulphite bismuth alcohol phenol red agar medium is described and has been found to allow rich growth of the true cholera vibrio and to inhibit the growth not only of B. coli and B. lactis aërogenes but of many vibrios liable to be mistaken for the true vibrio cholerae.

3. Thirty-one strains of true cholera vibrios, obtained from the National Collection, grew rapidly and profusely in the media.

4. Of twenty-five cholera-like and para-cholera strains six grew well, whilst the growth of nineteen was scanty or nil.

5. Of eleven El Tor vibrios five grew in both fluid and solid bismuth media, five grew only on the solid medium and in one no growth occurred in either medium. The El Tor colonies were much smaller than those of the epidemic cholera strains.

Type
Research Article
Information
Journal of Hygiene , Volume 40 , Issue 5 , September 1940 , pp. 532 - 537
Copyright
Copyright © Cambridge University Press 1940

References

REFERENCES

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