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A bacteriocin produced by certain M-type 49 Streptococcus pyogenes strains when incubated anaerobically

Published online by Cambridge University Press:  19 October 2009

John R. Tagg  and
Stephen A. Skjold
John R. Tagg
Affiliation:
Department of Microbiology, University of Otago, Dunedin, New Zealand
Stephen A. Skjold
Affiliation:
Departments of Pediatrics and Microbiology, University of MinnesotaMedical School, Minneapolis, Minnesota 55455, U.S.A.
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Bacteriocin production (P)-typing of 75 M-type 49 group-A streptococci obtained from a variety of epidemiological incidents in different countries gave no evidence of production under the usual aerobic test conditions. However, with anaerobic incubation, 28% of the strains gave a pattern of inhibitory activity against the indicator strains which was indistinguishable from that previously attributed to the bacteriocin, streptococcin A-FF22 (SA-FF22). Isolation and partial purification of the M type 49 bacteriocin (SA-M49) by freeze–thaw elution from anaerobically grown lawn cultures, followed by ammonium sulphate precipitation and Sephadex chromatography, showed the activity to be associated with a heat-stable proteinaceous molecule of molecular weight approximately 8000 – properties similar to those of SA-FF22. SA-FF22 and SA-M49 were found to have identical inhibitory spectra including immunity of the producer strains to the inhibitory activity of both the homologous and heterologous bacteriocin preparations. SA-M49 production occurred in some strains of phage subtypes II, III and provisional VI and. since it was a consistent property for all isolates from single outbreaks of infection, it provides a means of discriminating between strains of each of these three phage subtypes. There was no evidence of any increased incidence of SA-M49 production in M-type 49 strains associated with nephritic sequelae

Type
Research Article
Information
Journal of Hygiene , Volume 93 , Issue 2 , October 1984 , pp. 339 - 344
Copyright
Copyright © Cambridge University Press 1984

References

Johnson, D. W., Tagg, J. R. & Wannamaker, L. W. (1979). Production of a bacteriocine-like substance by group A streptococci of M-type 4 and T-pattern 4. Journal of Medical Microbiology 12, 413–427.CrossRefGoogle Scholar
Mayon-White, R. T. & Perks, E. M. (1982). Why type streptococci? The epidemiology of group A streptococci in Oxfordshire 1970–1980. Journal of Hygiene 88, 439–452.Google Scholar
Maxted, W. R., Fraser, C. A. M. & Parker, M. T. (1967). Streptococcus pyogenes, type 49. Lancet i 641–644.Google Scholar
Skjold, S. A. & Wannamaker, L. W. (1976). Method for phage typing group A type 49 streptococci. Journal of Clinical Microbiology 4, 232–238.CrossRefGoogle Scholar
Skjold, S. A., Wannamaker, L. W., Johnson, D. R. & Margolis, H. S. (1983). Type 49 Streptococcus pyogenes: phage subtypes as epidemiological markers in isolates from skin sepsis and acute glomerulonephritis. Journal of Hygiene 91, 71–76.Google Scholar
Simpson, W. J. & Tagg, J. R. (1983). M-type 57 group A streptococcus bacteriocin. Canadian Journal of Microbiology. 29, 1445–1452.CrossRefGoogle ScholarPubMed
Tagg, J. R. & Bannister, L. V. (1979). ‘Fingerprinting’ β-haemolytic streptococci by their production of and sensitivity to bacteriocine-like inhibitors. Journal of Medical Microbiology 12, 397–411.CrossRefGoogle ScholarPubMed
Tagg, J. R. & Martin, D. R. (1984). Evaluation of a typing scheme for group A streptococci based upon bacteriocin-like inhibitor production. Zentralblatt für Bakteriologie, Parasitenkunde, Infectionskrankheiten und Hygiene (Abt. I, Originale A) (In the Press).CrossRefGoogle Scholar
Tagg, J. R. & Wannamaker, L. W. (1978). Streptococcin A-FF22: Nisin-like antibiotic substance produced by a group A streptococcus. Antimicrobial Age7its and Chemotherapy 14, 31–39.CrossRefGoogle ScholarPubMed
Tagg, J. R., Dajani, A. S. & Wannamaker, L. W. (1976). Bacteriocins of Gram-positive bacteria. Bacteriological Reviews 40, 722–756.CrossRefGoogle ScholarPubMed
Tagg, J. R., Dajani, A. S., Wannamaker, L. W. & Gray, E. D. (1973 a). Group A streptococcal bacteriocin. Production, purification and mode of action. Journal of Experimental Medicine 138, 1168–1183.CrossRefGoogle Scholar
Tagg, J. R., Read, R. S. D. & McGiven, A. R. (1973 b). Bacteriocin of a group A streptococcus: partial purification and properties. Antimicrobial Agents and Chemotherapy. 4, 214–221.CrossRefGoogle Scholar