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The bactericidal properties of compounds which protect living cells against freezing damage

Published online by Cambridge University Press:  15 May 2009

T. Nash
T. Nash
Affiliation:
National Institute for Medical Research, Mill Hill, London, N. W. 7
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Most air disinfectants fail at low relative humidity. Those which remain active appear to be the same compounds which, in other circumstances, can protect living cells against the lethal damage caused by freezing to low temperatures and then thawing. The compounds are either simple glycols, whose respective activities in the two fields are already known, or the weaker Lewis bases, whose air disinfectant properties have not hitherto been investigated. It is suggested that both freezing protection and bactericidal action are due to the special solvent properties of these compounds, which come into play when most of the water usually present has been removed, by freezing or by drying respectively.

I am indebted to Dr 0. M. Lidwell for the loan of air sampling equipment, and to Dr L. N. Owen and to the Shell Chemical Co. for the gift of various diols.

Type
Research Article
Information
Journal of Hygiene , Volume 60 , Issue 3 , September 1962 , pp. 353 - 358
Copyright
Copyright © Cambridge University Press 1962

References

Bourdillon, R. B., Lidwell, O. M. & Lovelock, J. E. (1948 a). Studies in air hygiene. Spec. Rep. Ser., med. Res. Coun., Lond., no. 262. H.M.S.O.Google Scholar
Bourdillon, R. B., Lidwell, O. M. & Lovelock, J. E. (1948 b). Technique of bacterial killing tests. Studies in air hygiene. Spec. Rep. Ser., med. Res. Coun., Lond. no. 262, p. 54, H.M.S.O.Google Scholar
Bronsted, J. N. (1938). Solubility relations of high molecular weight substances. C.R. Lab. Carlsberg (Ser. chim.), 22, 99.Google Scholar
Coulson, C. A. (1957). The hydrogen bond—a review of the present position. Research, Lond., 10, 149.Google Scholar
Lester, W., Robertson, O. H., Puck, T. T., Wise, H. & Smith, M. (1949). The rate of bactericidal action of triethylene glycol vapor on micro-organisms dispersed into the air in small droplets. Amer. J. Hyg. 50, 175.Google Scholar
Lidwell, O. M. & Lovelock, J. E. (1948). Certain general features in air disinfection by chemicals. Studies in air hygiene. Spec. Rep. Ser., med. Res. Coun., Lond., no. 262, p. 147. H.M.S.O.Google Scholar
Lidwell, O. M., Lovelock, J. E. & Raymond, W. F. (1948). Studies in air hygiene. Spec. Rep. Ser., med. Res. Coun., Lond. no. 262, p. 75. H.M.S.O.Google Scholar
Lovelock, J. E. (1953). The haemolysis of human red-blood cells by freezing and thawing. Biochim. biophys. Acta, 10, 414.CrossRefGoogle ScholarPubMed
Lovelock, J. E. (1954). The protective action of neutral solutes against haemolysis by freezing and thawing. Biochem. J. 56, 265.CrossRefGoogle ScholarPubMed
Lovelock, J. E. & Bishop, M. W. H. (1959). Prevention of freezing damage to living cells by dimethyl sulphoxide. Nature, Loud., 183, 1394.CrossRefGoogle ScholarPubMed
Morgan, W. T. J. (1937). The isolation and properties of a specific antigenic substance from B. dysenteriae (Shiga). Biochern. J. 34, 2003.CrossRefGoogle Scholar
Nash, T. (1951). Physical aspects of air disinfection. J. Hyg., Camb., 49, 382.Google ScholarPubMed
Nash, T. (1961). Prevention of freezing damage to living cells by pyridine N-oxide. Nature, Loud., 192, 360.CrossRefGoogle ScholarPubMed
Nash, T. (1962 a). The solublity of organic non-electrolytes in water. (To be published.)Google Scholar
Nash, T. (1962 b). The chemical constitution of compounds which protect living cells against freezing damage. (Submitted for Publication.)CrossRefGoogle Scholar
Puck, T. T. (1947). The mechanism of aerial disinfection by glycols and other chemical agents. J. exp. Med., 85, 729.CrossRefGoogle ScholarPubMed
Robertson, O. H., Appel, E. M., Puck, T. T., Lemon, H. M. & Ritter, M. H. (1948). A study of the bactericidal activity in vitro of certain glycols and closely related compounds. J. infect. Dis. 83, 125.CrossRefGoogle ScholarPubMed