Hostname: page-component-7479d7b7d-jwnkl Total loading time: 0 Render date: 2024-07-12T00:47:28.234Z Has data issue: false hasContentIssue false
  • English
  • Français

Aspects of the epidemiology of bovine tuberculosis in badgers and cattle. II. The development and use of a typing system for Mycobacterium bovis

Published online by Cambridge University Press:  25 March 2010

P. A. Barrow
P. A. Barrow
Affiliation:
Department of Medical Microbiology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A relatively simple method for the detection of intra-specific variation of Mycobacterium bovis is described, based on the ability of strains to utilize or remove amino acids from solution. Using the method, 31 strains of M. bovis, isolated from badgers and cattle, have been divided into four major groups with some additional sub-groups and intermediate strains. Preliminary results suggest that the biotypes observed are relatively stable. The method was used in the investigation of an outbreak of bovine tuberculosis in cattle in Gloucestershire where badgers were implicated as a source of infection. The results suggested that transmission of infection had occurred on at least two or three separate occasions. It is suggested that the method could be used in studying the epidemiology of bovine tuberculosis in wild animals, cattle and man.

Type
Research Article
Information
Journal of Hygiene , Volume 86 , Issue 3 , June 1981 , pp. 247 - 257
Copyright
Copyright © Cambridge University Press 1981

References

REFERENCES

Baess, I. (1969). Sub-division of M. tuberculosis by means of bacteriophages. Acta Pathologica Microbiologica Scandinavica 76, 464–74.CrossRefGoogle Scholar
Barrow, P. A. & Gallagher, J. (1981). Aspects of the epidemiology of bovine tuberculosis in badgers and cattle. 1. The prevalence of infection in two wild animal populations in the south-west of England. Journal of Hygiene 86, 237–45.CrossRefGoogle Scholar
Gallagher, J. & Horwill, D. M. (1977). A selective oleic acid albumin agar medium for the cultivation of Mycobacterium bovis. Journal of Hygiene 79, 155–60.CrossRefGoogle ScholarPubMed
Grange, J. M. (1976). Enzymic breakdown of amino acids and related compounds by suspensions of washed mycobacteria. Journal of Applied Bacteriology 41, 425–31.CrossRefGoogle ScholarPubMed
Parker, M. T. (1972). Phage-typing of Staphylococcus aureus. In Methods in Microbiology 7B, (ed. Norris, J. R. and Ribbons, D. W.). London: Academic Press.Google Scholar
Redmond, W. B., Bates, J. H. & Engel, H. W. B. (1979). Methods for bacteriophage typing of mycobacteria. Methods in Microbiology 13 (ed. Bergan, T. and Norris, J. R.). London: Academic Press.Google Scholar
Report (1976, 1977 & 1979). Bovine tuberculosis in badgers. Unpublished reports by the Ministry of Agriculture, Fisheries & Food.Google Scholar
Richards, W. D. (1974). Phage susceptibilities of Mycobacterium bovis isolates. Annali Sclavo 17, 618–22.Google Scholar
Takeya, K. & Tokiwa, H. (1974). Bacteriocin-typing of Mycobacterium tuberculosis. American Review of Respiratory Diseases 109, 304–5.Google ScholarPubMed
Wieten, G., Haverkamp, J. & Meuzelaar, H. L. C. (1979). Pyrolysis mass spectrometry in mycobacterial taxonomy and identification. Proceedings of the VI Conference on Systematics of the Genus Mycobacterium, Atlanta, Georgia.Google Scholar
Wieten, G., Haverkamp, J., Meuzelaar, H. L. C., Engel, H. W. B. & Berwald, L. G. (1981). Use of pyrolysis mass spectrometry in the routine differentiation of mycobacteria of the ‘Tuberculosis complex’. Journal of Clinical Microbiology. (In the Press.)CrossRefGoogle Scholar
Williams, R. E. O. & Rippon, J. E. (1952). Bacteriophage typing of Staphylococcus aureus. Journal of Hygiene 50, 320–53.CrossRefGoogle ScholarPubMed