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Antimicrobial susceptibility and genetic characteristics of Haemophilus influenzae isolated from patients with respiratory tract infections between 1987 and 2000, including β-lactamase-negative ampicillin-resistant strains

  • L. QIN (a1), H. WATANABE (a2), N. ASOH (a2), K. WATANABE (a2), K. OISHI (a2), T. MIZOTA (a1) and T. NAGATAKE (a2)...

Summary

The minimum inhibitory concentration (MIC) of five antibiotics and the presence of resistance genes was determined in 163 Haemophilus influenzae isolates collected over 13 years (1987–2000) in four two-yearly sampling periods from patients with respiratory tract infections. The prevalence of β-lactamase-negative ampicillin-susceptible strains was approximately 80% over the sampling period although fewer strains (65·9%) were recovered in the period 1995–1997. TEM-1 type β-lactamase-producing strains were less frequent starting at 15·6% and declining to 2·2% in the final sampling period. Low-β-lactamase-negative ampicillin-resistant (BLNAR) strains were uncommon in 1987–1989 (2·2%), peaked to 19·5% in 1995–1997, but fell back to 11·1% by 2000. Fully BLNAR strains were not detected until the last sampling period (6·7%). The MICs of ampicillin, levofloxacin, cefditoren and ceftriaxone remained stable but there was an eight-fold increase in the MIC of cefdinir over the sampling period. Pulsed-field gel electrophoresis of DNA digests showed that three representative BLNAR strains were genetically distinct and 11 DNA profiles were identified among 17 low-BLNAR strains. These data suggest that the number of genetically altered BLNAR and low-BLNAR strains are increasing in Japan.

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Copyright

COPYRIGHT: © Cambridge University Press 2006

Corresponding author

*Author for correspondence: Dr H. Watanabe, Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. (Email: h-wata@net.nagasaki-u.ac.jp)

References

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1. Murphy, TF, Apicella, MA. Nontypeable Haemophilus influenzae: a review of clinical aspects, surface antigens, and the human immune response to infection. Reviews of Infectious Diseases 1987; 9: 1–15.
2. Saito, M, et al. . Clonal spread of an invasive strain of Haemophilus influenzae type b among nursery contacts accompanied by a high carriage rate of non-disease-associated strains. Journal of Medical Microbiology 2000; 49: 845–847.
3. Felmingham, D, Washington, J. Trends in the antimicrobial susceptibility of bacterial respiratory tract pathogens – findings of the Alexander Project 1992–1996. Journal of Chemotherapy 1999; 11: 5–21.
4. Karlowsky, JA, et al. . Antimicrobial surveillance of Haemophilus influenzae in the United States during 2000–2001 leads to detection of clonal dissemination of a beta-lactamase-negative and ampicillin-resistant strain. Journal of Clinical Microbiology 2002; 40: 1063–1066.
5. Hoban, D, Felmingham, D. The PROTEKT surveillance study: antimicrobial susceptibility of Haemophilus influenzae and Moraxella catarrhalis from community-acquired respiratory tract infections. Journal of Antimicrobial Chemotherapy 2003; 50: 49–59.
6. Suzuki, K, Nishimura, T, Baba, S. Current status of bacterial resistance in the otolaryngology field: results from the Second Nationwide Survey in Japan. Journal of Incfection and Chemotherapy 2003; 9: 46–52.
7. Ubukata, K, et al. . Association of amino acid substitutions in penicillin-binding protein 3 with beta-lactam resistance in beta-lactamase-negative ampicillin-resistant Haemophilus influenzae. Antimicrobial Agents and Chemotherapy 2001; 45: 1693–1699.
8. Hasegawa, K, et al. . Rapidly increasing prevalence of β-lactamase-nonproducing, ampicillin-resistant Haemophilus influenzae type b in patients with meningitis. Antimicrobial Agents and Chemotherapy 2004; 48: 1509–1514.
9.National Committee for Clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7-A4. Wayne, PA: National Committee for Clinical Laboratory Standards, 1998.
10. Yano, H, et al. . Pulsed-field gel electrophoresis analysis of nasopharyngeal flora in children attending a day care center. Journal of Clinical Microbiology 2000; 38: 625–629.
11. Tenover, FC, et al. . Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. Journal of Clinical Microbiology 1995; 33: 2233–2239.
12. Heilmann, KP, et al. . Decreasing prevalence of beta-lactamase production among respiratory tract isolates of Haemophilus influenzae in the United States. Antimicrobial Agents and Chemotherapy 2005; 49: 2561–2564.
13. Dabernat, H, et al. . Haemophilus influenzae carriage in children attending French day care centers: a molecular epidemiological study. Journal of Clinical Microbiology 2003; 41: 1664–1672.
14. Marco, F, et al. . Antimicrobial susceptibilities of 1,730 Haemophilus influenzae respiratory tract isolates in Spain in 1998–1999. Antimicrobial Agents and Chemotherapy 2001; 45: 3226–3228.
15. Seki, H, et al. . Increasing prevalence of ampicillin-resistant, non-beta-lactamase-producing strains of Haemophilus influenzae in children in Japan. Chemotherapy 1999; 45: 15–21.
16. Watanabe, H, et al. . Possible high rate of transmission of nontypeable Haemophilus influenzae, including β-lactamase-negative ampicillin-resistant strains, between children and their parents. Journal of Clinical Microbiology 2004; 42: 362–365.
17. Rennie, RP, Ibrahim, KH. Antimicrobial resistance in Haemophilus influenzae: how can we prevent the inevitable? Commentary on antimicrobial resistance in H. influenzae based on data from the TARGETed surveillance program. Clinical Infectious Diseases 2005; 41 (Suppl. 4): S234–S238.
  • Cited by 4

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Antimicrobial susceptibility and genetic characteristics of Haemophilus influenzae isolated from patients with respiratory tract infections between 1987 and 2000, including β-lactamase-negative ampicillin-resistant strains

  • L. QIN (a1), H. WATANABE (a2), N. ASOH (a2), K. WATANABE (a2), K. OISHI (a2), T. MIZOTA (a1) and T. NAGATAKE (a2)...

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