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Analysis of methicillin-resistant and methicillin-susceptible Staphylococcus aureus by a molecular typing method based on coagulase gene polymorphisms

Published online by Cambridge University Press:  15 May 2009

N. Kobayashi ,
K. Taniguchi ,
K. Kojima ,
S. Urasawa ,
N. Uehara ,
Y. Omizu ,
Y. Kishi ,
A. Yagihashi  and
I. Kurokawa
N. Kobayashi
Affiliation:
Department of Hygiene, School of Medicine, Sapporo Medical University, S-l W-17, Chuo-ku,Sapporo 060, Japan
K. Taniguchi
Affiliation:
Department of Hygiene, School of Medicine, Sapporo Medical University, S-l W-17, Chuo-ku,Sapporo 060, Japan
K. Kojima
Affiliation:
Department of Hygiene, School of Medicine, Sapporo Medical University, S-l W-17, Chuo-ku,Sapporo 060, Japan
S. Urasawa
Affiliation:
Department of Hygiene, School of Medicine, Sapporo Medical University, S-l W-17, Chuo-ku,Sapporo 060, Japan
N. Uehara
Affiliation:
Department of Laboratory Diagnosis, School of Medicine, Sapporo Medical University, S-1, W-16, Chuo-ku, Sapporo 060, Japan
Y. Omizu
Affiliation:
Department of Laboratory Diagnosis, School of Medicine, Sapporo Medical University, S-1, W-16, Chuo-ku, Sapporo 060, Japan
Y. Kishi
Affiliation:
Department of Laboratory Diagnosis, School of Medicine, Sapporo Medical University, S-1, W-16, Chuo-ku, Sapporo 060, Japan
A. Yagihashi
Affiliation:
Department of Laboratory Diagnosis, School of Medicine, Sapporo Medical University, S-1, W-16, Chuo-ku, Sapporo 060, Japan
I. Kurokawa
Affiliation:
Department of Laboratory Diagnosis, School of Medicine, Sapporo Medical University, S-1, W-16, Chuo-ku, Sapporo 060, Japan
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A molecular typing method for Staphylococcus aureus based on coagulase gene polymorphisms (coagulase gene typing) was evaluated by examining a total of 240 isolates which comprised 210 methicillin-resistant S. aureus (MRSA) and 30 methicillin-susceptible S. aureus (MSSA) collected from a single hospital. By Alul restriction enzyme digestion of the PCR-amplified 3′-end region of the coagulase gene including 81-bp repeated units, the MRSA and MSSA isolates examined were divided into 6 and 12 restriction fragment length polymorphism (RFLP) patterns, respectively, whereas five patterns were commonly detected in MRSA and MSSA. MRSA isolates that showed a particular RFLP pattern were considered to be predominant in the hospital. Coagulase typing with type-specific antisera was also performed for all S. aureus isolates for comparison. Coagulase types II and VII were most frequently detected and included isolates with four and five different AluI RFLP patterns, respectively, whereas each of the other coagulase types corresponded to a single RFLP pattern. These results indicated that RFLP typing was more discriminatory than serological typing, for typing S. aureus and demonstrated its utility in epidemiologic investigation of S. aureus infection in hospitals.

Type
Research Article
Information
Epidemiology & Infection , Volume 115 , Issue 3 , December 1995 , pp. 419 - 426
Copyright
Copyright © Cambridge University Press 1995

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